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- EPIK™ miRNA Select Lo-ROX Kit
The EPIK miRNA Select Assay Kit contains any four individual EPIK miRNA RT-qPCR assays (100 reactions per assay). Choose from between 1 and 4 different assays per kit. Assays designed, validated upon order will require an additional two weeks delivery time and assays designed and validated upon order will require an additional three weeks delivery time.
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The unique priming of the EPIK™ miRNA Assays confers distinct advantages over other approaches to miRNA detection. EPIK miRNA Assays use modified stem-loop, miRNA-specific oligonucleotide mediated reverse transcription and hemi-nested real-time PCR, combined with SensiSMART™ and SYBR® Green, offering remarkable sensitivities as well as extremely low background, thereby enabling the accurate detection of very low input miRNA levels and accurate discrimination of even very closely related miRNA targets.
Using advanced design concepts, a proprietary algorithm has been developed by MiRXES™ to create modified stem-loop miRNA specific reverse transcription primers and hemi-nested real-time PCR primer combinations, to maximize miRNA detection sensitivity while minimizing non-specific interactions. Each assay is evaluated using over 36 design parameters before being extensively wet-lab validated using both synthetic miRNA template and reference human RNA. The assays use SYBR® Green rather than a probe-based system for detection, allowing rapid amplification when required (Fig. 1). The resulting real-time PCR assays use SensiSMART™ enabling detection of extremely low levels of miRNA with high specificity, allowing the discrimination between closely related miRNA sequences (Fig. 2).
Performance of these assays have been validated to detect as few as 100 copies of template per RT reaction (Fig. 3) with excellent assay efficiency and linearity, to produce a list of individual miRNA assays that are ideal for validation and determination of miRNA function.
The EPIK miRNA Select Assays comprise a choice from over 27,000 miRNAs listed in the current version of miRBase. Each kit contains primers specific for up to four miRNAs along with reverse transcriptase and sufficient SensiSMART for 400 real-time PCR reactions. If you cannot find the miRNA that you are interested in, please inquire..
EPIK miRNA Assays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
|20x RT Primers||4 x 16 µl|
|10x PCR Primers||4 x 200 µl|
|EPIK RT Buffer||1 x 160 µl|
|EPIK RT Enzyme||1 x 40 µl|
|2x SensiSMART™ Lo-ROX Master Mix||4 x 1 ml|
|DEPC Water||5 x 1.8 ml|
All components are shipped on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.