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    Description

    CH3-Blue Chemically Competent Cells are provided at a transformation efficiency of 1x108 cfu/µg supercoiled DNA, making them ideal for difficult clone construction or blunt end ligation.

    Product Highlights

    • Versatile -cloning of methylated and non-methylated DNA
    • High efficiency -available in ≥108 cfu/μg, perfect for difficult clone construction
    • Easy screening -with blue/white color selection
    • Convenient size -small aliquotes reduce waste

    Product Description

    CH3-Blue Chemically Competent Cells are a highly efficient derivative of E. coli K12, ideal for difficult clone construction using plasmid derived vectors.

    To facilitate the cloning of DNA that contains methylcytosine or 5-hydroxymethylcytosine, CH3-Blue lacks the E.coli restriction systems mcrA, mcrBC, mrr and hsdRMS.

    The addition of endA1 allows for cleaner preparations of DNA and better results in downstream applications. The elimination of non-specific digestion by Endonuclease I and the recA1 reduces the occurrence of non-specific recombination in cloned DNA. This minimizes recombination events and improves the quality and yield of plasmid DNA.

    The lacZ mutation allows blue/white color screening and α-complementation of recombinants.

    Applications

    • Cloning of methylated DNA
    • Ideal for subcloning
    • Generating cDNA libraries
    • Blue/white color screening

    Resources

    Documents

     

    Certification of Analysis (COAs)

    Specification

    Components

    Reagent

    BIO-85039

    Chemically Competent Cells

    10 x 100 µL

    pUC19 control vector (10 pg/µL)

    100 µL

    Storage & Stability

    All kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.

    Shipping conditions

    CH3-Blue 1x108 Chemically Competent Cells are shipped on dry/blue ice



    FAQs

    No, we currently do not have a product for recombinant protein production.

    No, all of our offered cells have a dam plus genotype.