| Sign In | Quick Order CART (0)
  • Total RNA

    The ISOLATE II range includes kits designed for the isolation and purification of total RNA from a wide variety of samples, including samples that are sometimes difficult to process. The kits are supplied with filters (shredders) and DNase where required, to enhance sample homogenization and remove genomic DNA. The ISOLATE II range does not incorporate organic solvents, thereby improving safety and avoiding bias in recovery between different RNA species.

    The ISOLATE II RNA Kits have been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST™ cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Mini Kit can be used to purify samples for PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq™ DNA Polymerase.

    • Fast – simple extraction of high purity total RNA ideal for use in all applications
    • High-performance – recovery of consistently high quality RNA from a wide variety of samples
    • Efficient – optimized lysis conditions and column matrix for maximum recovery of high purity RNA
    • Convenient – includes all necessary components, including filters (shredders) and DNase I
    • Safe - no hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl or alcohol precipitation

    ISOLATE II Guide

    Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your research

    ISOLATE II Selection Chart

    Select the best reagent for your research based on your sample requirements

    Working with RNA Hints and Tips

    Hints and tips to help you maintain an RNase-free environment

    “We have been utilizing ISOLATE II RNA Mini Kit from Bioline and have found that the RNA yield, concentrations and purity have been consistently good. From 40,000 liver cancer (mouse and human) cells we are able to get 200-600 ng/ul yield of RNA.”

    Aparna Jayachandran, GMRF-UQ, Brisbane, Australia

    Micro RNA

    The ISOLATE II range also incorporates kits for simple, efficient column-based method for the isolation of miRNA and total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol, which has been reported to introduce significant bias in recovery of selected small RNAs.

    The ISOLATE II miRNA Kit has been designed to deliver optimal performance with the EPIK™ Panel and Select Assays in miRNA expression profiling and quantification using qPCR, microarrays, small RNA library construction for small RNA-Seq, as well as single cell assays.

    • Fast – streamlined protocols for reliable, rapid extraction of all miRNA species from a wide variety of sample types
    • Specific – buffering conditions and column matrix specially developed for extraction of the miRNA population
    • Efficient – excellent recovery of all small RNA species (<200 nt) from even very limiting amounts of sample
    • Unbiased – column-based purification eliminates bias in the small RNA molecule population that can occur with organic solvents
    • Versatile – delivers high-purity miRNA from a variety of sample types including cells, tissue, bacteria and bodily fluids.
    • Convenient - optional protocol for parallel extraction of all larger RNA species thereby enabling extraction of total RNA

    Genomic DNA

    The ISOLATE II portfolio incorporates kits for the rapid purification of genomic DNA from a broad range of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded tissue (FFPE), stool viruses, bacterial DNA, biological fluids or clinical specimens, bacterial DNA and viral DNA from urine, insects, dental swabs and buccal swabs.

    All ISOLATE II Kits for genomic DNA purification employ highly-efficient column-based without the need for hazardous reagents such as phenol, thereby improving safety and circumventing any bias in recovery of different DNA species. The ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

    • Fast – streamlined protocols for consistent recovery of genomic DNA
    • High-performance – extraction of high-purity DNA, ideal for use in all downstream applications
    • High-yield – efficient sample lysis and column-binding for high-yield recovery of genomic DNA from even very limiting amounts of sample
    • Flexible – reliable extraction of genomic DNA from a very broad range of human samples

    Plasmid DNA

    The ISOLATE II portfolio includes a kit for simple, rapid, efficient recovery of plasmid DNA from even low bacterial culture volumes, without the need for hazardous reagents such as phenol. Separate protocols are provided for the column-based purification of high-copy plasmids, low-copy plasmid, P1 constructs and cosmid DNA from E. coli as well as the purification of plasmids from gram-positive bacteria.

    PCR Clean Up

    Rapid, simple, efficient membrane-based purification of DNA from PCR reactions and from TAE and TBE agarose gels, as well as PCR reactions, without the need for hazardous reagents or ethanol precipitation. The ISOLATE II PCR and Gel Kit is ideal for purifying DNA for use in transformations, cloning, sequencing and restriction analysis.

    Column-free Purification

    TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials. By combining thiocyanate compounds with phenol, TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population.

    SureClean Plus provides a simple, efficient method for the purification and concentration of DNA or dsRNA from PCR reactions or any enzymatic digests, without the need for hazardous reagents. SureClean Plus removes primers, primer-dimers, dNTPs and proteins. SureClean Plus also includes a pink co-precipitant that can be added to the sample to facilitate easy visualization of the purified pellet.

    Reviews

    "I recently used the ISOLATE II RNA Mini kit. I had some cultured cells which are ..."

    "We used the Isolate II RNA Micro Kit, SensiFAST cDNA synthesis kit and ..."

    "I found the ISOLATE II RNA/DNA/Protein Kit very easy to use, the sample ..."

    "We have been utilizing ISOLATE II RNA Mini Kit from Bioline and have found ..."

    "I have used this kit to extract genomic DNA from mice tails (approx. 0.6 cm) for ..."

    "The ISOLATE II Genomic DNA Kit is extremely sensitive. It produced ..."