ISOLATE II Biofluids RNA Kit

Cat No. Size List Price* Qty  
BIO-52086 50 Preps $274.00
 
ADD TO CART
*For more information on pricing please contact us

Add to favorite products Email to a Friend Print View
ISOLATE II Biofluids RNA Kit is developed for the rapid, phenol-free isolation of high quality and high yield total RNA from a wide range of biofluids including blood, serum, plasma, urine, saliva, CSF as well as RNA viruses.

Features & Benefits

  • High quality total RNA - efficient recovery from challenging biofluids and viral samples
  • High purity and yield - ideal for samples with low RNA content
  • Complete RNA diversity - all sizes of RNA isolated, from large mRNA, viral RNA down to miRNA and siRNA
  • Rapid, phenol-free protocol (20 min/10 preps) - increase your throughput
  • DNase I included - efficient digestion of genomic DNA for sensitive applications

Applications

Isolation of all sizes of RNA from:
  • Biofluids including blood, serum, plasma, urine, saliva, CSF
  • Viruses
Pure, isolated RNA ready for all downstream applications such as real-time RT-PCR, RNA-seq and microarrays.

Description

The ISOLATE II Biofluids RNA Kit is specially developed for the rapid isolation of high quality total RNA from cell-free biofluids and viruses. High yields of total RNA can be purified from blood (fig. 1), plasma, serum and other types of biofluids such as saliva, urine, semen and cerebrospinal fluid. Viral RNA can be isolated from the same sample sources, as well as cultured cells and tissue. The kit isolates all sizes of RNA from large mRNA, viral RNA and ribosomal RNA down to small RNAs such as miRNA and siRNA. The ISOLATE II Biofluids RNA Kit is compatible with very limited, or challenging sample sources containing low RNA content.

Isolation is based on a fast spin column format using a novel RNA affinity silica as the separation matrix. RNA molecules are preferentially purified from other cellular components such as proteins without the use of phenol or chloroform.

Samples are first lysed in the presence of guanidinium thiocyanate, a chaotropic salt which immediately deactivates endogenous RNases to ensure purification of intact RNA. DNA is then captured on a Genomic DNA Removal Column to remove contaminating genomic DNA. Ethanol is added to the flow-through and then applied to a RNA Column. The bound total RNA is washed to remove any impurities and eluted.

The purified RNA is of the highest purity and integrity and is ready for use in various applications, including Real-Time RT-PCR, RT-PCR, cDNA synthesis, RNA-seq and microarrays.


Components

Reagent 50 Reactions
ISOLATE II RNA Columns 50
ISOLATE II Genomic DNA Removal Columns 50
Collection Tubes (2ml) 100
Elution Tubes (1.7ml) 50 
Lysis Buffer RX 40ml
Wash Buffer W1 38ml
DNase I Solution (RNase-free) 0.8ml
DNase I Reaction Buffer DRB 6ml
RNA Elution Buffer 6ml
Product Manual 1
Bench Protocol Sheet 1

Storage & Stability

The DNase I Solution should be stored at -20°C upon arrival. All other components should be stored dry and at room temperature.

When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.


Shipping conditions

Ambient temperature







^ Back to Top