| Sign In | Quick Order CART (0)
  • Ordering

    Cat. No.
    Size
    List Price*
    QTY
    BIO-52062
    10 Preps
    $37.50
    +
    BIO-52064
    250 Preps
    $540.00
    +
    *To Check your pricing please Login or contact us

    Description

    Highly-efficient purification of genomic DNA from whole blood, cultured cells, serum, plasma or other body fluids by combining the stringency of guanidinium thiocyanate lysis with the speed and purity of silica-membrane column purification.

    Product Highlights

    • Fast – simple extraction of high purity genomic DNA, ideal for use in all applications, in as little as 30 minutes
    • High-performance – recovery of consistently high quality genomic DNA from whole blood, cultured cells, serum, plasma and other body fluids
    • Efficient – optimized lysis conditions and column matrix for improved recovery of genomic DNA from each sample
    • Versatile – recovery of host genomic DNA, bacterial and viral DNA from human and animal blood samples
    • Safe - no hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl or alcohol precipitation

    Product Description

    The ISOLATE II blood DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from blood, without the need for hazardous reagents such as phenol.

    By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Blood DNA Kit provides a fast method for the purification of high-quality genomic DNA from whole blood, cultured cells, serum, plasma and other human / animal body fluids treated with EDTA, citrate or heparin. It is also possible to purify bacterial and viral DNA (e.g. HBV) from blood samples.

    The ISOLATE II Blood DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR with any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

    Applications

    • qPCR
    • End-point PCR
    • Multiplex PCR
    • Southern, dot and slot blotting
    • Genotyping
    • Next Generation Sequencing
    • Genetic testing
    Main
    Fast purification of high-quality genomic DNA from whole blood, cultured cells, serum, plasma or other body fluids.

    Nucleic Acid Isolation Guide

    Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your research

    Resources

    Specification

    Components

    Reagent

    10 Preps

    50 Preps

    250 Preps

    ISOLATE II Blood DNA Spin Columns & Collection Tubes

    10

    50

    250

    Collection Tubes (2 ml)

    20

    100

    500 

    Buffer G1

    3.2 mL

    10 mL

    50 mL

    Buffer G2

    0.8 mL

    2.5 mL

    12.5 mL

    Wash Buffer GW1

    6 mL

    30 mL

    2 x 75 mL

    Wash Buffer GW2

    4 mL

    7 mL

    2 x 20 mL

    Elution Buffer G

    4 mL

    13 mL

    60 mL

    Proteinase K (lyophilized)

    6 mg

    30 mg

    2 x 75 mg

    Proteinase K Buffer PR

    0.8 mL

    1.8 mL

    8 mL

    Labels for Lysis Buffer G3

    1

    1

    1

    Product Manual

    1

    1

    1

    Bench Protocol Sheet

    1

    1

    1

    Storage & Stability

    All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.

    Shipping conditions

    Ambient temperature.



    FAQs

    The columns supplied with the ISOLATE II kits may appear similar, but each type has been optimized to work within the buffer system supplied with the corresponding kit. The swapping of columns (or buffers) between kits may lead to no recovery of nucleic acid whatsoever, or at the very least severely impaired purification.

    Interruption of the DNA/RNA extraction process is possible after the sample lysis only. It is possible to homogenize and lyse the samples and to store them in the freezer until use for RNA extraction. RNA clean up with a column cannot be interrupted and we recommend to avoid delays during the column purification process. If a delay is unavoidable the columns should be stored on ice.

    The elution buffer is ideal for applications such as restriction enzyme digestion, sequencing and PCR. It is possible to use DNase-free water for elution, but you should expect a slightly lower yield.

    The elution buffer of this kit does not contain EDTA which could interfere with downstream applications like PCR or sequencing.

    It is not recommended to exceed the maximum volume to prevent clogging of the column. It is possible to split the sample and work with several columns in parallel.