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    BIO-68025
    16 Reactions
    $720.00
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    Description

    The success of next-generation sequencing is dependent upon the precise and accurate processing of the input DNA. This requires high quality library preparation from sheared DNA and relies on a coordinated series of standard molecular biology reactions whilst maintaining high yields during the intermediate purification steps.

    Product Highlights

    • Low input: end-repair, A-tailing and ligation combined in the same tube, thereby eliminating cleanup steps and improving sample yield
    • Improved confidence: simpler automatable protocol with fewer steps for reducing the risk of sample loss, offering greater peace-of-mind
    • Increased speed: library preparation in less than 3 hours for reduced time-to-results and increased sample throughput
    • Highly efficient: reaction buffer pre-optimized to provide maximum reaction efficiency and highest conversion rates
    • Improved quality: reliable library preparation from even the most challenging samples providing maximum coverage
    • Maximum convenience: sequencing adaptors, enabling indexing of up to 16 samples, already included in the kit

    Product Description

    The success of next-generation sequencing is partly dependent upon the precise and accurate processing of the input DNA. High-quality library preparation from sheared DNA requires efficient processing during a series of molecular biology reactions and good recovery during the intermediate purification steps. Bioline has developed an optimized, single-tube library construction method (fig. 1) that enables fast and highly efficient adaptor ligated library construction from a range of sample types and inputs.

    The combination of end repair and A-tailing followed by direct ligation of the adaptors reduces the hands-on time, making the process faster and easier to use. Optimized end-repair, A-tailing and ligation steps and the reduction in cleanup steps also offer significant improvement in the yield and quality of sequence-ready libraries, reducing the amount of DNA required.

    The JetSeq DNA Library Preparation Kit is highly efficient at converting DNA into an adapter-ligated library resulting in higher sequence coverage. The kit contains all of the enzymes and buffers necessary for highly-efficient conversion of the input DNA to a sequencable, adapter-ligated library (fig. 2).

    The JetSeq DNA Library Preparation Kit uses a highly efficient polymerase, together with an optimized buffer formulation to ensure uniform amplification of all genomic regions including those that contain highly variable GC content, thereby reducing bias and ensuring even coverage in subsequent sequencing reactions, resulting in exceptional sequence quality scores even 150 bases into the read (fig. 3), giving greater coverage and reducing the possibility of sequencing gaps.

    Main

    Resources

     

    Certification of Analysis (COAs)

    Specification

    Components

    ER Buffer 160 µL
    ER Enzyme Mix 96 µL
    Ligation buffer 48 µL
    Adaptor A 80 µL
    Adaptor B 80 µL
    Ligase 32 µL
    PCR Buffer 80 µL
    Primer Mix 80 µL
    DNA Polymerase 32 µL
    PCR Buffer 80 µL
    Primer 16 µL
    DNA Polymerase 32 µL
    Nuclease Free Water 1.8 mL
    Index 1 - 16 20 µL

    Shipping conditions

    On Dry Ice or Blue Ice.



    FAQs

    As a general guideline, inputs of 10 ng or more are compatible with complex genomic DNA, cell-free DNA and low-quality DNA such as FFPE samples. For small genomes, cell-free DNA and lower complexity samples such as ChIP DNA, amplicons or cDNA library construction, sample amounts of approximately 100 pg or more may be attempted.

    Yes, it is possible. Degraded samples should not be a problem, but we recommend to assess the grade of degradation with a Bioanalyzer. Degraded or FFPE sample DNA may need higher template amount due to low quality.

    It is not currently possible with the JetSeq DNA Library Preparation Kit. The included adapter, indices and PCR primer are matched to each other and it is not possible to exchange one of them. For the JetSeq Flex DNA Library Preparation Kit and JetSeq ER and Ligation Kit, you will need to use your own adapters/indices, as they are not provided in the kit.