- Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets
- Reproducible – consistent results between technical replicates for increased confidence in results
- Robust – reliable detection of RNA targets from a broad range of sample types
- Fast – delivers reproducible, accurate assay results in as little as 40 minutes
- Efficient – excellent performance in multiplex assays
- Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
The SensiFAST Probe No-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays.
An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.
The SensiFAST™ Probe No-ROX One-Step Kit has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.
- Gene expression analysis
- Pathogen detection
- RNA viral pathogen detection
- Genetic profiling
- miRNA profiling / quantification
- Genetically modified organisms (GMO) characterization
Introduction to SensiFASTOverview, features and benefits of the SensiFAST product family
SensiFAST™ One-Step Real-Time PCR GuideDownload the SensiFAST One-Step Real-Time PCR Guide with detailed product descriptions and performance data to help you choose the best product for your research
One-step Vs. Two-step real-time RT PCRA discussion of the pros and cons of each detection strategy.
I compared the SensiFAST Probe One-Step Kit with another very popular product. SensiFAST Probe One-Step gave improved sensitivity, which was reflected in lower Ct values. Also, this product is a very good price. Value for money!!
Peony Fung, Cerberus Sciences, Thebarton, Australia
Instrument CompatibilityPlease refer to the Real-Time PCR Selection Chart to confirm the SensiFAST Probe No-ROX One-Step Kit is compatible with your instrument.
Certification of Analysis (COAs)
100 x 20 µL Reactions
500 x 20 µL Reactions
SensiFAST Probe No-ROX One-Step Mix (2x)
1 x 1 mL
5 x 1 mL
RiboSafe RNase Inhibitor
1 x 40 µL
1 x 200 µL
1 x 20 µL
1 x 100 µL
1 x 1.8 mL
2 x 1.8 mL
- BIO-76001: 100 x 20 µL Reactions: 1 x 1mL
- BIO-76005: 500 x 20 µL Reactions: 5 x 1mL
Storage & Stability
All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.
Shipped on dry/blue ice.
Human - Rosato R.R., et al. Cancer Res. 67: 9490-500 (2007)
Rat - Remund K., et al. Expt. Lung Res. 35: 359-370 (2009)
Drosophila - Brown A.E., et al. PloS ONE 4: e4490 (2009)
Nitrogen-fixing bacteria - Bahlawane C., et al. Mol. Plant-Microbe Inter. 21: 1498-1509 (2008)
Diphtheria bacteria - Jochmann N., et al. Microbiology 155: 1459-1477 (2009)
Green algae - Wobbe L., et al. PNAS 106: 13290-13295 (2009)
Bovine virus - Park S-I., et al. J. Virol. Methods. 159: 64-6 (2009)
For cDNA/DNA templates SensiFAST SYBR® and Probe kits are used. To give you some idea of a few of the types of templates used with these kits:
Stem Cell - Bernardo A.S., et al. Stem Cells 27(2): 341 -351 (2008)
Cow - Baumert A., et al. J. Dairy Research 76(3): 356-364 (2009)
Mouse - Hoyles R.K., et al. Arthritis Rheum. 58(4): 1175-88 (2008)
Quail - De Winter P., et al. British Poultry Science 49(5): 566 – 573 (2008)
Insect - Bass C., et al. Malaria journal 6 111 (2007)
Fish - Miller M.R., et al. J. Nutr. 138(11): 2179-2185 (2008)
Crab - Wilcockson D.C. & Webster S.G. Gen. & Comp. Endo. 156: 113–125 (2008)
Nematode - Murray S.L., et al. Mol. Plant-Microbe Interactions 20(11): 1431–1438 (2007)
Plant - Hecht V., et al. Plant Physiology Preview DOI:10.1104/pp.107.096818 (2007)
Yeast - Kawauchi J., et al. Genes & Dev. 22: 1082-1092 (2008)
E.coli Bacteria - Saeed H.A., et al. Research J. Microbiology 4(4); 173-177 (2009)
Virus - Muscillo M., et al. Water, Air, & Soil Pollution 191: 1-4 (2008)