- Sensitive – incorporates MyTaq DNA polymerase that exhibits increased affinity for DNA thereby improving amplification of even limiting amounts of template
- Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
- Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
- Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
- Convenient – buffer system includes a red dye for improved pipetting ease / accuracy and to enable direct gel loading
- Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
MyTaq™ Red DNA Polymerase is recommended for all standard PCR applications. The MyTaq DNA Polymerase and MyTaq Red Reaction Buffer in this product, are a unique combination of next-generation polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq means it gives excellent results under fast PCR conditions.
The enzyme is supplied with a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq Red Reaction Buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.
- Standard PCR
- High-yield PCR
- Fast PCR
- Colony PCR
- TA cloning
We use MyTaq Red DNA Polymerase weekly in the lab for our mouse genotyping. Fast, efficient, reliable and cost effective. Thanks Bioline!
Nora Tenis, SVI, Molecular Genetics, Fitzroy, AU
Product SelectionPlease refer to the PCR Selection Chart to confirm the recommended product for your PCR application.
Certification of Analysis (COAs)
MyTaq Red DNA Polymerase
1 x 100 µL
2 x 250 µL
4 x 250 µL
5x MyTaq Reaction Buffer
4 x 1 mL
14 x 1.5 mL
9 x 5 mL
Storage & Stability
MyTaq Red DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label.
On Dry Ice or Blue Ice.
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
|No or low PCR yield||Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.|
|Primers degraded – check quality and age of the primers.|
|Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM.|
|Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration.|
|Template concentration too low – Increase concentration of template.|
|Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.|
|Multiple Bands||Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.|
|Prepare master mixes on ice or use a heat-activated polymerase.|
|For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.|
|Smearing or artifacts||Template concentration too high. Prepare serial dilutions of template.|
|Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.|
|Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.|
|Extension time too long. Reduce extension time in 0.5-1 minute increments.|