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Description
Product Highlights
- Sensitive - reproducible detection of low-copy number templates
- Reproducible – consistent results between technical replicates for increased confidence in results
- Specific - proprietary hot-start modification minimizes non-specific amplification for improved assay reliability in high-throughput assays
- Robust – reliable, accurate detection of DNA and cDNA targets from a broad range of sample types
Product Description
The SensiMix™ SYBR® & Fluorescein Kit has been developed for highly accurate real-time PCR and has been validated on real-time PCR platforms that require the passive reference dye fluorescein for collection of dynamic well factors.
A proprietary, highly-optimized, chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix SYBR® & Fluorescein Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions.
The SensiMix SYBR® & Fluorescein Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.
Applications
- Gene expression analysis
- DNA / cDNA target detection
- miRNA profiling / quantification
- Copy number variation (CNV) analysis

SensiMix products from Bioline are easy to use and give consistent results, they make the switch to real-time PCR very simple.
Biobest Laboratories, Scotland, UK
Instrument Compatibility
Please refer to the Real-Time PCR Selection Chart to confirm the recommended product for your PCR application.Resources
Certification of Analysis (COAs)
Specification
Components
Reagent |
500 x 50 µL Reactions |
2000 x 50 µL Reactions |
SensiMix™ SYBR® & Fluorescein (2x) |
10 x 1.25 mL (12.5 mL) |
40 x 1.25 mL (50 mL) |
Volume
- QT615-05: 500 x 50 µL Reactions: 10 x 1.25 mL
- QT615-20: 2000 x 50 µL Reactions: 40 x 1.25 mL
Storage & Stability
All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.
Shipping conditions
Shipped on dry/blue ice.