| Sign In | Quick Order CART (0)
  • Ordering

    Cat. No.
    Size
    List Price*
    QTY
    BIO-85025
    2ml (10 x 200µl)
    $120.00
    +
    *To check your pricing please Login or contact us

    Description

    α-Select Bronze Competent Cells are ideal for routine subcloning.

    Product Highlights

    • Reduced recombination of cloned DNA - accommodates larger plasmids
    • High efficiency - available in ≥107 cfu/μg, perfect for routine subcloning
    • Easy screening - with blue/white color selection
    • Convenient size - small aliquots reduce waste

    Product Description

    α-Select Bronze Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for subcloning using plasmid-derived vectors. α-Select Bronze Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.

    pUC19 DNA is also provided as a positive control.

    Applications

    • Transformation of large plasmids into bacterial cells
    • Generating clones
    • Blue/white color screening

    Resources

     

    Certification of Analysis (COAs)

    Specification

    Components

    Reagent

    BIO-85025

    Chemically Competent Cells

    10 x 200 µL

    pUC19 control vector (10 pg/µL)

    100 µL

    Storage & Stability

    All kit components should be stored at -80°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.

    Shipping conditions

    Shipped on Dry Ice or Blue Ice.



    FAQs

    No, we currently do not have a product for recombinant protein production.

    No, all of our offered cells have a dam plus genotype.