Our R&D team is always there to help directly with any problems and queries. We take pride in our teams capabilities to produce fantastic results for our customers.
The Ct values are close to the negative because we were extracting the DNA in an inappropriately way. It is our fault. It is not a matter of the MasterMix. The mix works perfectly. We are using the SensiFAST Probe Lo-ROX Kit from Bioline.
Following your advice, today we repeated the DNA extraction leaving a little bit of the aqueous phase with the organic phase and the intermediate layer. To our surprise and happiness, the results were much better than the previous ones. We were able to detect DNA from bacteria infecting seeds at less than 1 UFC/ml of bacteria (we use 10 ml as starting material, which means approx. 7 cell per extraction tube).
And, What was the difference? this time we kept the interphase with the organic phase. In previous experiments during the DNA isolation we removed the interphase or part of it. If we keep this intermediate phase we get a final pellet very big (DNA and other remains) and not very clean. The samples that we process are from seeds (difficult samples) and we thought that the intermediate layer could contain PCR inhibitors. So, we decided to remove it. This time we decided to retain the intermediate layer with the organic phase to isolate the DNA. Things were much better and no PCR inhibitors were detected. Probably, we were losing a lot of DNA by removing the interphase.
The today results looks very promising even with difficult samples as seeds.
I want to thank you the time you have spent trying to solve our problem. Your help has been very valuable for us.
Dr Leandro de Leon, Anove Asociación
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"I have been at Bioline for seven months as the UK Sales Manager. The time has flown by, as sales jobs tend to do. Each day is different; some days out speaking with customers, learning about their research and matching their needs with our products; others are working with my colleagues internally, such as R&D and Marketing especially recently whilst we have been releasing our new products.
I speak to my team regularly. There are so many different personalities and they can complement one another well. Our focus is to sell the right product to the right customer at the right price.
Bioline is a great place to work. We are known for having good quality products that have helped many people through their studies over the years. Recently, we have focused on providing our reagents for In vitro Diagnostic kits, having received ISO13485 accreditation at our manufacturing plant. We are always looking for new opportunities."
Miles Collier, UK Sales Manager
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The success of next-generation sequencing is dependent upon the precise and accurate processing of the input DNA. This requires high quality library preparation from sheared DNA and relies on a coordinated series of standard molecular biology reactions whilst maintaining high yields during the intermediate purification steps.
ISO 13485:2012 is the International standard against which the quality management and quality assurance system of the business is evaluated.
This certification is central to Bioline’s customer commitment to be the leading provider of Molecular Biology Reagents and to set the standard for excellence across the business in this field.
Our ISO certification brings a new level of confidence to your decision making which our current and future partners and customers can rely upon.
Currently ISO 9001:2008 is the International standard against which the quality management and quality assurance system is certified for the design, manufacture, distribution and customer support services at these Bioline locations.
Our focus for continuous improvement and increased customer satisfaction, is now leading us towards re-certification to the medical device quality management system of ISO 13485:2012. This commitment to quality will provide the re-assurance to our customers of the best service and products from one of the world’s leading molecular biology reagents companies.
"Working as a research and development scientist at Bioline has allowed me to make the projects I work on my own. It has allowed me to combine my imagination together with concrete evidence to produce promising and outstanding products.
As the junior scientist on the team my time at Bioline has allowed me to grow in my technical knowledge as well as make the most of my potential. It has given me the opportunity to experience working in a professional environment and be part of a highly qualified team.
I love working at Bioline! Ever since I started it has been an incredible journey. I learn something new everyday and feel that with each passing day I am one step closer to becoming a better scientist.
We are a friendly and high standard company always keeping up with the latest trends and technologies. Everyone is always ready to help making Bioline a pleasant place to work.
For the last year I have been working on my own project which as been an invaluable experience. I hope to be part of many more projects to come."
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