Thank you Rebecca Traub from University of Melbourne, for a great review on our ISOLATE Fecal DNA Kit
"Aim: To compare the sensitivity of two commercial faecal DNA extraction kits for the detection and quantification of human hookworms (Ancylostoma ceylanicum, Ancylostoma duodenale and Necator americanus) eggs in faeces.
Methods: Ten individual human faecal samples positive for hookworm eggs were each extracted with two commercial kits, the Powersoil DNA Isolation Kit (MoBio) and the ISOLATE Fecal DNA Kit (Bioline) according to manufacturer's instructions. DNA was eluted to 100 ul for each.
DNA was subjected to multiplex Taq-man based multiplex qPCR (unpublished) targeting the internal transcribed spacer regions of the three human hookworms and an internal control (EHV4).
Results: Both kits performed equivocally in positively detecting at least one species of hookworm in all ten samples. The ISOLATE Fecal DNA Kit produced on average 0.5 Ct lower threshold cycles for samples compared to the Powersoil DNA Isolation Kit. The time taken to extract 10 samples was significantly less for the Bioline product.
Conclusion: The Powersoil DNA Isolation Kit and the ISOLATE Fecal DNA Kit are comparable in terms of sensitivity for detecting and quantifying DNA of hookworms from human faecal samples. The Bioline kit was less labour intensive compared to the Mo Bio kit and is more competitively priced."
Click the link to view the data >> Hookworm Multiplex Kit Data
Click the link to learn more about >> ISOLATE Fecal DNA Kit
"I am a Business Development Manager (Asia) based out of Singapore office. My role is to find the right partners in Asia and to work very closely with them to build Bioline brand ing name and expand the business in these regions. Being in this role involves a lot of travel all around Asia and it is great to experience the different cultures, meet various customers and understand the vast differences in politics, customs and business dealings in various parts of Asia. I love to be on the field together with my partners to engage in discussion with our customers. It is gratifying when we have a fruitful discussion and managed to find the right solution to meet our customers’ needs and expectations. Asia is a rapidly evolving region which offers both fresh challenges and big opportunities. I am all up to for taking on the challenge and am excited to be part of the Bioline team.
Away from Bioline, I have another job. A job that I don’t get paid for but is very rewarding, I’m a mummy to a 2.5 years old little girl named Eliza. As I travel quite a bit in my job, I value my family time very much. I spend my off days doing things that we enjoy together as a family such as going to the zoo, cycling, rock climbing, swimming and having an impromptu picnic or outing. It is not easy to strike a perfect balance between work and family, but I try my best to achieve a satisfying, fulfilling and well-balanced life both personally and professionally."
Fadlina Musa, Business Development Manager
#testimonial #BiolineTeam #WomenInSTEM
Thank you Jonathan Ferrand from Hudson Institute of Medical Research, VIC Australia, for a great review on our MyTaq™ Red Mix
"We have been using the MyTaq™ Red Mix for the past two years in our routine PCRs for mice genotyping, cloning/amplicon validation and standard curve preparation for our RTqPCR. This product is very convenient with gel loading buffer included, and in our hands work as well as competitor products with as low as 5ul of Mix per reaction (10ul final volume). The competitive pricing makes it a very attractive alternative for routine PCRs."
Click the link to learn more about >> MyTaq™ Red Mix