Agarose gel electrophoresis is a common molecular biology technique used to separate DNA or RNA molecules by size. This is achieved by denaturing and applying a negative charge to our nucleic acid sample. The samples are then run through an agarose matrix with an electric field (Electrophoresis). Shorter molecules move faster and thus migrate further and in the gel over a given time period. Many factors are involved with regards to the final visualization step and achieving high resolution of the resulting bands on the agarose gel.
Tips to help improve resolution include: - Running the gel at a lower voltage for a longer period of time - Using a wider/thinner gel comb - Loading less DNA into each well
MangoTaq comes with a coloured reaction buffer that contains red and orange dyes, which separate during electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel.
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