Thank you, Benson Lindsey from The Ohio State University, for a great review on our MyTaq Plant Direct PCR Kit.
"We tested the MyTaq Plant Direct PCR Kit in our Arabidopsis genotyping workflow. It saved us a lot of time by cutting out the tedious isolation of genomic DNA from leaves. We got consistent results using both the direct leaf protocol and the difficult samples protocol using SDS. We found that lysed leaf samples in SDS could be stored at -20 oC and reused multiple times over several weeks, with no discernible loss of quality. We also were able to use MyTaq Plant Direct PCR Kit to 'rescue' genomic DNA, resulting from poor quality isolations that would not amplify with other polymerases.
MyTaq Plant Direct PCR Kit performed better than the competing product we tried. While both kits provided comparable amplification in a 50 mL reaction, only the MyTaq Plant Direct PCR Kit was able to amplify directly from leaf tissue in a 25 mL reaction."