Thank you B.Dhungel, from Gallipoli Medical Research Foundation (GMRF), Greenslopes Private Hospital, University of Queensland for such a great review.
I am really impressed by the reproduciblity of the Bioline Isolate II RNA minikit, the cDNA sysnthesis and the Sybr-Lo Rox mix in qPCR. We consistently get high concentrations of pure RNA with 260/280 reading 2 when measured with the nanodrop.
We have seen that the cDNA synthesis kit from Bioline is able to synthesize high quality cDNA even from low starting RNA concentrations when analyzing the qPCR results.
Based on the latest developments in silica membrane spin column technology and optimized buffer chemistry, with simple bind wash elute steps; the new Bioline ISOLATE II Purification Kits are designed for rapid, phenol free isolation of highest quality nucleic acids, including miRNA, even from samples that are difficult to process. By using ISOLATE II Kits, researchers can expect superior yields, higher purity and integrity when compared to the previous generation of purification kits, as well as safe and convenient handling without the use of toxic chemicals.
Improved and unbiased recovery
All five kits offer significantly improved and unbiased recovery of small RNA molecules, including miRNA and siRNA, to give highly accurate results. In contrast, widely used phenol-chloroform based methods involve laborious separation and precipitation steps and can introduce significant bias in small RNA recovery. The new kits also efficiently purify high-quality large RNA species such as mRNA.
Marco Calzavara, President of Bioline commented,
“With the additional five kits to the ISOLATE II nucleic acid purification range, researchers can achieve the purity and yield necessary for unbiased cDNA synthesis, high performance real time RT PCR and highly accurate RNA sequencing. This makes ISOLATE II the perfect starting point for a wide array of downstream applications including miRNA expression profiling and quantification, biomarker discovery, viral screening, pathogen detection, and agribiotech research."
The five new ISOLATE II miRNA and RNA Purification Kits are available now for a wide range of starting samples and tissue types.