Quantifying nucleic acids by Real-Time qPCR
4th-5th September 2013, Birkbeck, University of London
Register now for a two-day, hands-on workshop sponsored by Bioline: The PCR Company that teaches you the core principles of qPCR and focuses on the practical steps required to design, set-up, validate and analyse Real-Time qPCR assays.
The 6th International Meeting on Synthetic Biology (hashtag: #SB6Conf), the world’s foremost synthetic biology (SynBio) meeting, is currently running in London, organised by the BioBricks Foundation, a non-profit organization based in Cambridge, Massachusetts.
BioBricks Foundation SB6.0 Co-Chairs include Professor Paul Freemont and Professor Richard Kitney of Imperial College, who will lead a new £10 million innovation and knowledge centre, to be called SynbiCITE, aimed at providing a bridge between academia and industry and announced at the conference by David Willetts, Minister for Universities and Science.
Imperial College is home to the UK Centre for Synthetic Biology and Innovation a £9m investment aimed at propelling the synthetic biology field forward and promoting SynBio start-ups. International collaboration and networking are important aspects of the meeting, along with poster presentations and ‘lightning talks’ from world leaders in the field of SynBio research.
David Willets said:
"Synthetic biology has huge potential for our economy and society in so many areas, from life sciences to agriculture. But to realise this potential we need to ensure researchers and business work together. This new Innovation and Knowledge Centre will help advance scientific knowledge and turn cutting edge research into commercial success."
Professor Richard Kitney, co-academic of SynbiCITE added:
"Synthetic Biology could be the next ‘industrial revolution’ for the UK, where tiny devices manufactured from cells are used by us to improve many facets of our lives. From producing new, more sustainable fuels to developing devices that can monitor or improve our health, the applications in this field are limitless."
The exciting and emerging field of Synthetic Biology research combines the disciplines of engineering and molecular biology to design and build novel, biologically-based parts, devices, and sensors, as well as the re-engineering of existing, natural biological organisms. Synthetic Biology has the potential to deliver important new applications, from detecting the early onset of disease and improving existing industrial processes, food production, green fuels, and developing therapies to fight harmful bacterial infections or cancers.
Much of the future success of synthetic biology is incumbent upon the development of standardized SynBio components that can be combined in predictable and repeatable ways. The precise approach used when fabricating a BioBrick component depends on the fabrication method (PCR or direct synthesis) as well as the type of part being constructed (a standard part or protein coding sequence).
SyntheticBiology.org maintains a nice introduction to Synthetic Biology as well as a useful how-to guide on the subject of constructing novel BioBrick parts for submission to the Registry of Standard Biological Parts.
Bioline makes it easy to harness the power of new generation enzymes to create parts for BioBricks using PCR. We manufacture and supply a range of high-performance PCR and molecular biology cloning tools, enabling researchers to drive their synthetic biology projects forward. Some of our most popular, most frequently used products for leading synthetic biologists presenting at SB6.0 (1, 2) include the High-Fidelity Velocity DNA Polymerase, MyTaq HS DNA Polymerase for Colony-PCR, Competent Cells, Quick-Stick Ligase for TA Cloning, ISOLATE II Plasmid Mini Kits, and our acclaimed range of SensiMix™ and SensiFAST™ Real-Time PCR kits.
If you've been attending the #SB6Conf in London this week, let us know if your poster cites any Bioline reagents and your synthetic biology research work and achievements could be showcased in a forthcoming SynBio article on the Bioline blog. And if you’re one of the IGEMers attending the conference, don’t forget to check out our Gem of an offer for iGEM Teams!
One final note regarding the future of synthetic biology and the synthetic biologists of the future, you can keep up to date with all the latest news from the iGEM SynBio research teams around the world by following the international iGEM Teams Twitter list maintained by us @ThePCRCompany.
1. Giuraniuc CV, MacPherson M, Saka Y, et al. (2013). Gateway Vectors for Efficient Artificial Gene Assembly In Vitro and Expression in Yeast Saccharomyces cerevisiae. PLoS ONE 8(5): e64419. doi:10.1371/journal.pone.0064419
2. Ali H, Ries MI, Nijland JG, Lankhorst PP, Hankemeier T, et al. (2013). A Branched Biosynthetic Pathway Is Involved in Production of Roquefortine and Related Compounds in Penicillium chrysogenum. PLoS ONE 8(6): e65328. doi:10.1371/journal.pone.0065328
Australia Day traditionally marks the anniversary in 1788, when Captain Arthur Philip and the First Fleet arrived at Port Jackson, now part of Sydney, New South Wales, to establish the first European settlement. The holiday is an opportunity for Australians to celebrate the founding of the country and its culture.
Bioline is also privileged to call Sydney home. For 10 years, Bioline Australia has been proud to be a primary manufacturer and supplier of best-in-class molecular biology tools to the leading research universities and institutes in Australia. Bioline's new R&D facility in the Australian Technology Park in Sydney was officially opened by the Hon. Verity Firth MP (Minister for Science and Medical Research) in November 2007. For those interested, photos from the launch are available here.
We thought the Australia Day celebrations might be a good time to highlight some of the scientific achievements of our antipodean cousins, so this month's Bioline Scholar features a selection of interesting, recently published papers from scientists 'down under'.
Blyton M.D. et al. (2013). High temporal variability in commensal Escherichia coli strain communities of an herbivorous marsupial. Environ. Microbiol. doi: 10.1111/1462-2920.12088
In this study from The Australian National University in Canberra, commensal E. coli strains of mountain brushtail possums were quantified at both the host population level and within individuals. E. coli strains were identified using rep-PCR profiling and quadruplex PCR. A high level of temporal variability was found.
Luter, H.M. et al. (2012). Thermal and sedimentation stress are unlikely causes of brown spot syndrome in the coral reef sponge, Ianthella basta. PLoS ONE 7(6): e39779.
Brown spot lesions currently affect a large population of I. basta, an ecologically important sponge species on the Great Barrier Reef. Researchers from the Australian Institute of Marine Science at James Cook University found changes in the microbial community of I. basta to be stable. Thermal and sedimentation stress were not likely to be responsible for the syndrome.
Kelly, R.D. et al. (2013). Mitochondrial DNA haplotypes define gene expression patterns in pluripotent and differentiating embryonic stem cells. Stem Cells doi: 10.1002/stem.1313
In this paper from the Monash Institute of Medical Research in Melbourne, the effects of different mitochondrial DNA haplotypes on differentiation and development were studied using embryonic stem cell lines. In conclusion, mitochondrial DNA haplotypes play a pivotal role in the process of differentiation and mediate cell fate.
Roberts, T. et al. (2013). Subtype distribution of Blastocystis isolates from a variety of animals from New South Wales, Australia. Vet. Parasit. doi: 10.1016/j.vetpar.2013.01.011
Researchers from St Vincent's Hospital and the University of Technology, Sydney identified nine different genetic subtypes of Blastocytis, a genus of Protozoan parasite, in different animal species in NSW. Blastocystis was identified for the first-time from the eastern grey kangaroo, red kangaroo, wallaroo, snow leopard and ostrich. Further investigation into the genetic diversity of Blastocystis may help identify the zoonotic potential of Blastocystis.
Covelloa, J.M. et al. (2013). Isolation of RAG-1 and IgM transcripts from the striped trumpeter (Latris lineata), and their expression as markers for development of the adaptive immune response. Fish Shellfish Immun. doi: 10.1016/j.fsi.2012.12.015
In this work from the National Centre for Marine Conservation and Resource Sustainability, University of Tasmania, mRNA sequences from the RAG-1 and IgM heavy chain were analysed from the striped trumpeter (Latris lineata). The expression of the two genes was assessed throughout the early developmental stages of striped trumpeter larvae and used as markers to follow the ontogeny of the adaptive immune response.
That's it for this edition of Bioline Scholar! If you have published research using Bioline products, then get in touch, tell us and your paper could make it into a future edition of Bioline Scholar.
Bioline is proud to announce the latest addition to its family of SensiFAST Real-Time PCR products.
SensiFAST™ HRM Kit facilitates High Resolution Melt (HRM) curve analysis, enabling amplification and discrimination of even the most challenging sequence differences -such as class 4 SNPs- without sequence preference.
SensiFAST HRM is designed to deliver fast, accurate detection of gene mutations and SNPs and provides reliable and highly reproducible data on all commonly used real-time PCR instruments, especially the new generation of fast-cyclers. SensiFAST HRM does not require expensive labelled oligonucleotide probes and offers a cost effective alternative to traditional probe based genotyping methods.
Marco Calzavara, President of Bioline said:
For more information, please visit the SensiFAST™ HRM product page.
This year’s BIOTECHNICA was a great success for Bioline, with lots of visits and product enquiries from new and existing customers. We would like to thank those of you who took the time to come and speak with us.
We received great feedback from visitors who listened to Senior Global Product Manager Dr. Steve Hawkins' talk on Wednesday 12th October, Use of DNA and RNA Extraction Controls in Real-Time PCR. The talk heralded the launch of Bioline's DNA (DEC) and RNA Extraction Control (REC) products for Real-Time PCR at the Innovation Forum and created a definite buzz with lots of interest from attendees, particularly from those doing in-house diagnostics. We also had a lot of interest in our popular SensiFAST™ Real-Time PCR product range and MyTaq™ DNA polymerase family.
The iPad2 competition at the stand was also extremely popular. The lucky winner will be contacted shortly by Holger Berthel, our Sales Manager in Germany, so good luck to everyone who entered!