Thank you, Tennille Sibbritt from the Children's Medical Research Institute - Embryology Unit, for a great review on our SensiFAST™ SYBR® No-ROX Kit.
"I am preparing samples for RNA-seq using a protocol that has been optimised for low input samples. I performed a qPCR on two housekeeping genes (Actb and Tbp) as a quality control step to ensure good amplification of these genes before proceeding to library preparation. I initially performed the qPCR using a combination of Taq and SYBR, which led to Ct values of 20-22 for Tbp and 23-26 for Actb. I repeated this qPCR using the 200 L sample of SensiFAST SYBR No ROX master mix I received. This worked very well and was quite sensitive. The Ct values for Tbp were 19-20 and for Actb were 17-19, while the no template control had minimal amplification. I plan on using SensiFAST SYBR No ROX master mix in the future, especially since it is substantially cheaper than what I was previously using."
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Thank you, Joey Liu from Ohio State University, for a great review on our SensiFAST™ SYBR® No-ROX Kit.
"The performance of the SensiFAST™ SYBR® No-ROX Kit is robust. We have used it with ABI 7900 and Roche LightCycler 480 for gene expression for a while. The studies include a variety of human and mouse primary cells and cell lines. We are very happy with the kit because of its sensitivity and repeatability and will continue to go with it."
Click here to learn more about >> SensiFAST™ SYBR® No-ROX Kit