Bioline offers over 27,000 miRNAs, covering all organisms in the miRBase, designed using the proprietary algorithm developed by developed by MiRXES™ to maximize miRNA detection sensitivity, while minimizing non-specific interactions.
The assays use SYBR® Green rather than a probe-based system for detection, allowing rapid amplification when required. The resulting real-time PCR assays enable detection of extremely low levels of miRNA with high specificity, allowing the discrimination between closely related miRNA sequences.
All validated EPIK™ miRNA Select Assays are tested using both synthetic miRNA templates and total RNA. Typically the assays detect as few as 100 copies of template per RT reaction with excellent assay efficiency and linearity.