Ordering
Description
Product Highlights
- Fast – streamlined protocol for extraction of host cell and microbial DNA in as little as 15 minutes
- High-performance – recovery of consistently high-quality DNA, ideal for use in all downstream applications
- Efficient – rapid and effective lysis of fecal samples by bead beating for excellent recovery of DNA
- Flexible – reliable recovery of host and microbial DNA from human and a broad range of animal species
Product Description
The ISOLATE II Fecal DNA Kit provides a simple, efficient column-based method for the isolation of DNA from a wide variety of fecal samples, without the need for hazardous reagents such as phenol.
By combining the stringency of guanidinium-thiocyanate lysis and high-density bashing beads and the speed and ease-of-use of silica-membrane purification to remove humic acids/polyphenols, the ISOLATE II Fecal DNA Kit provides a fast method for the purification of high-quality bacterial/protist DNA from a variety of fecal samples including humans, birds, rats, mice, rabbits, cattle, etc. as well as host DNA.
The ISOLATE II Fecal DNA Kit has been designed to purify samples ideal for use in end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase and in qPCR using the the SensiFAST Real-Time PCR Kits.
Applications
- End-point PCR
- qPCR
- Southern, dot and slot blotting
- Genotyping
- Next Generation Sequencing
- Bisulfite conversion/methylation analysis
Nucleic Acid Isolation Guide
Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your researchProduct Selection
Please refer to the ISOLATE II Selection Chart to confirm the applications for which the ISOLATE II Fecal DNA Kit is recommended.Resources
Documents
Certification of Analysis (COAs)
Specification
Components
|
Reagent |
50 Preps |
100 Preps |
|
Lysis Buffer |
1 x 40 mL |
2 x 40 mL |
|
DNA Pre-Wash Buffer |
1 x 15 mL |
2 x 15 mL |
|
Fecal DNA Wash Buffer |
1 x 50 mL |
2 x 50 mL |
|
Fecal DNA Binding Buffer |
1 x 100 mL |
2 x 100 mL |
|
DNA Elution Buffer |
1 x 10 mL |
2 x 10 mL |
|
Fecal Prep Solution |
1 x 30 mL |
2 x 30 mL |
|
Bashing Beads Lysis Tubes |
1 x 50 |
2 x 50 |
|
Spin II A Filters |
1 x 50 |
2 x 50 |
|
Spin II B Filters |
1 x 50 |
2 x 50 |
|
Spin Columns |
1 x 50 |
2 x 50 |
|
Collection Tubes |
200 |
2 x 200 |
|
Bench Protocol Sheet |
1 |
1 |
Storage & Stability
All components should be stored dry and at room temperature. When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.
Shipping conditions
Ambient temperature.
