Ordering
Cat. No.
Size
BIO-52082
50 Preps
The ISOLATE II Fecal DNA Kit provides a simple, efficient column-based method for the isolation of DNA from a wide variety of fecal samples, without the need for hazardous reagents such as phenol.
By combining the stringency of guanidinium-thiocyanate lysis and high-density bashing beads and the speed and ease-of-use of silica-membrane purification to remove humic acids/polyphenols, the ISOLATE II Fecal DNA Kit provides a fast method for the purification of high-quality bacterial/protist DNA from a variety of fecal samples including humans, birds, rats, mice, rabbits, cattle, etc. as well as host DNA.
The ISOLATE II Fecal DNA Kit has been designed to purify samples ideal for use in end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase and in qPCR using the the SensiFAST Real-Time PCR Kits.
|
Reagent |
50 Preps |
100 Preps |
|
Lysis Buffer |
1 x 40 mL |
2 x 40 mL |
|
DNA Pre-Wash Buffer |
1 x 15 mL |
2 x 15 mL |
|
Fecal DNA Wash Buffer |
1 x 50 mL |
2 x 50 mL |
|
Fecal DNA Binding Buffer |
1 x 100 mL |
2 x 100 mL |
|
DNA Elution Buffer |
1 x 10 mL |
2 x 10 mL |
|
Fecal Prep Solution |
1 x 30 mL |
2 x 30 mL |
|
Bashing Beads Lysis Tubes |
1 x 50 |
2 x 50 |
|
Spin II A Filters |
1 x 50 |
2 x 50 |
|
Spin II B Filters |
1 x 50 |
2 x 50 |
|
Spin Columns |
1 x 50 |
2 x 50 |
|
Collection Tubes |
200 |
2 x 200 |
|
Bench Protocol Sheet |
1 |
1 |
All components should be stored dry and at room temperature. When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.
Ambient temperature.
