Spectrophotometric Measurement of Nucleic Acid Concentration

This calculator is used to calculate the concentration of nucleic acids according to their optical density. Can be used for ssDNA, dsDNA, RNA and oligonucleotide samples. It is better to use {OD260-OD320} instead of OD260. For a series of measurements (batch mode) you should insert in the "Absorbance OD260" field the optical densities separated by a space.


 - choose for the "batch mode"

 [µg/ml] - extinction coefficient
it would be better to use special for oligonucleotide

Dilution volume:   µl
Sample volume:   µl
Absorbance OD260:  


Results for the "batch mode":