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Description
Product Highlights
- Fast – single-tube protocol that eliminates wash steps, giving high-yield, PCR-ready DNA in just 15 minutes
- Simple – few protocol steps greatly reduce the risk of sample loss and contamination and minimizes manual effort
- Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving yield of even the most challenging targets
- Specific - MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
- Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from mammalian tissue samples
- Convenient – mastermix facilitates PCR set-up and includes a red dye for improved pipetting ease / accuracy and to enable direct gel loading
Product Description
Many DNA extraction methods can be laborious and time consuming or involve the use of hazardous chemicals. MyTaq™ Extract-PCR Kit offers a rapid, easy and safer alternative for the extraction and amplification of DNA from a variety of tissue types. MyTaq Extract-PCR Kit is particularly suited to solid tissues such as mouse tail or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination.
The extracted DNA is amplified in a proprietary buffer system using MyTaq HS Red Mix, the latest generation of very high-performance polymerase unique to Bioline. To further reduce non-specific amplification, MyTaq HS uses antibody hot-start technology. The advanced formulation of MyTaq HS Red Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield.
The rapid MyTaq Extract-PCR Kit maximizes sensitivity while minimizing contamination risks to deliver improved success rates in applications such as mouse DNA characterization. The single tube lysis protocol and MyTaq HS Red Mix maximize sensitivity, while minimizing contamination risks and significantly reduce reaction times as well as delivering improved success rates in protocols such as mouse DNA characterization
Applications
- High-throughput genotyping from mammalian tissue
- Detection of transgenes
- Knockout analysis
Introduction to MyTaq
Overview, features and benefits of the MyTaq product family
PCR Enzyme Guide
Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your researchApplication Note
MyTaq™ Extract-PCR KitWe tested the MyTaq Extract-PCR Kit for genotyping mice. The extraction was fast, easy to handle and the PCR reactions worked very well. We used the Taq also for performing multiplex PCR and we obtained better results than with our conventional method.
Michael Mitterer, MPI of Immunobiology and Epigenetics, Freiburg
Product Selection
Please refer to the PCR Selection Chart to confirm the recommended product for your PCR application.Resources
Documents
Certification of Analysis (COAs)
Specification
Components
|
Reagent |
100 Reactions |
500 Reactions |
|
Buffer A |
2 x 1 mL |
10 x 1 mL |
|
Buffer B |
1 x 1 mL |
5 x 1 mL |
|
MyTaq HS Red Mix, 2x |
1 x 1.25 mL |
5 x 1.25 mL |
Concentration
2x
QC specifications
Bioline operates under ISO 13485 Management System. MyTaq Extract-PCR Kit and its components are extensively tested for activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination prior to release.
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
Shipping conditions
Shipped on Blue Ice.
FAQs
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
| Observation | Recommended Solution(s) |
| No or low PCR yield | Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments. |
| Primers degraded – check quality and age of the primers. | |
| Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM. | |
| Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration. | |
| Template concentration too low – Increase concentration of template. | |
| Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated. | |
| Multiple Bands | Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers. |
| Prepare master mixes on ice or use a heat-activated polymerase. | |
| For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity. | |
| Smearing or artifacts | Template concentration too high. Prepare serial dilutions of template. |
| Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands. | |
| Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments. | |
| Extension time too long. Reduce extension time in 0.5-1 minute increments. |
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