- Sensitive – incorporates MyTaq HS DNA Polymerase which exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
- Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
- Specific – MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
- Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
- Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
- Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast conditions
MyTaq™ HS DNA Polymerase is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme, specifically designed for highly-specific, efficient amplification from even the most challenging templates. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start.
The MyTaq HS DNA Polymerase and MyTaq Reaction Buffer in this product are a unique combination of next-generation hot-start polymerase and novel buffer system, that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases, meaning it performs reliably even in the presence of PCR inhibitors. Furthermore, the advanced formulation of MyTaq HS and buffer system allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield.
MyTaq Reaction Buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.
- Fast PCR
- Multiplex PCR
- Specific amplification of challenging (e.g. GC-rich) templates
- Colony PCR
- Low copy number PCR assays
- High-throughput assays with prolonged PCR set-up
Introduction to MyTaq
Overview, features and benefits of the MyTaq product family
PCR Selection ChartSelect the best reagent for your research
PCR Enzyme GuideDownload the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research
Application NoteMyTaq™ HS DNA Polymerase
MyTaq HS DNA Polymerase is more robust than the other enzymes I’ve tried.
Sydney University, Save Sight Institute, Sydney, Australia
Product SelectionPlease refer to the PCR Selection Chart to confirm the recommended product for your PCR application.
Certification of Analysis (COAs)
MyTaq HS DNA Polymerase
1 x 50 µL
1 x 200 µL
2 x 250 µL
5x MyTaq Reaction Buffer
2 x 1 mL
8 x 1 mL
14 x 1.5 mL
Storage & Stability
All components are shipped on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.
Components may also be stored at +4°C if required, although storage at -20°C is recommended. When stored at +4°C, reagents will remain stable for a period of 2 weeks from date of receipt.
On Dry Ice or Blue Ice.
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
|No or low PCR yield||Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.|
|Primers degraded – check quality and age of the primers.|
|Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM.|
|Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration.|
|Template concentration too low – Increase concentration of template.|
|Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.|
|Multiple Bands||Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.|
|Prepare master mixes on ice or use a heat-activated polymerase.|
|For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.|
|Smearing or artifacts||Template concentration too high. Prepare serial dilutions of template.|
|Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.|
|Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.|
|Extension time too long. Reduce extension time in 0.5-1 minute increments.|