- Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
- Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
- Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
- Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
- Convenient – an all-in-one-tube mastermix and red dye, that improves the speed, convenience and accuracy of PCR set-up
- Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling condition
MyTaq™ HS Red Mix is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. MyTaq HS Red Mix is comprised of MyTaq HS DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq HS means it gives excellent results under fast PCR conditions. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start.
The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up for improved speed, throughput and assay reproducibility. The inclusion of dNTPs, MgCl2 and enhancers at optimal concentrations, helps eliminate the need for optimization, thereby saving time and ensuring comparable efficiencies for annealing and extension of all primers in the reaction, this makes MyTaq HS Red Mix ideal for multiplex PCR.
MyTaq HS Red Mix, includes a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR eliminating the need to add loading buffer.
- Fast PCR
- Multiplex PCR
- Complex templates (e.g. GC-rich)
- Colony PCR
- Low copy number PCR assays
- High-throughput assays with prolonged PCR set-up
Introduction to MyTaq
Overview, features and benefits of the MyTaq product family
PCR Enzyme GuideDownload the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research
PCR Selection ChartSelect the best reagent for your research
Application NoteMyTaq™ HS Red Mix
I use the MyTaq HS Red Mix all the time for genotyping purposes. This polymerase is so efficient that my program for 35 cycles finishes just under one hour. The mix is easy to use, and the dye in it allows for direct gel loading after the PCR is finished.
Camilla Teng, University of Southern California, Los Angeles, US
Product SelectionPlease refer to the PCR Selection Chart to confirm the recommended product for your PCR application.
Certification of Analysis (COAs)
MyTaq HS Red Mix, 2x
4 x 1.25 mL
20 x 1.25 mL
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
On Dry Ice or Blue Ice.
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
|No or low PCR yield||Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.|
|Primers degraded – check quality and age of the primers.|
|Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM.|
|Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration.|
|Template concentration too low – Increase concentration of template.|
|Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.|
|Multiple Bands||Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.|
|Prepare master mixes on ice or use a heat-activated polymerase.|
|For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.|
|Smearing or artifacts||Template concentration too high. Prepare serial dilutions of template.|
|Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.|
|Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.|
|Extension time too long. Reduce extension time in 0.5-1 minute increments.|