Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room temperature.
Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to improve enzyme activity and which contains a specially developed 4x Quick-Stick Buffer.
- Fast - dramatically decreases time for DNA cloning
- Catalyzes the joining of double-stranded DNA - for blunt or cohesive ends
- Compatible with downstream applications - no loss of transformation efficiency
Quick-Stick Ligase is designed to carry out fast and efficient ligation of both cohesive and blunt-ended DNA at room temperature. Quick-Stick Ligase is a T4 DNA Ligase that has been mutated to improve enzyme activity, and contains a specially developed 4x Quick-Stick Buffer. The enzyme catalyses the joining of two strands of DNA between the 5’-phosphate and the 3’-hydroxyl groups of adjacent nucleotides in either a blunt-ended or cohesive-ended configuration.
Quick-Stick Ligase will ligate 99% of λ/HindIII cohesive-ended fragments, or 80% of λ/EcoRV blunt-ended fragments, in 5 minutes at room temperature. 100% ligation of blunt-ended fragments can be achieved by increasing the ligation time to 15 minutes at room temperature.
- Cloning of DNA from:
- PCR products
- Genomic, phage and viral DNA
- Linker ligation
- Re-ligation of linearized plasmids
- Ligation of double-stranded oligonucleotides into vectors (plasmid and phage)
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Certification of Analysis (COAs)
1 x 50 µL
4 x QS Buffer
1 x 250 µL
DNA Dilution Buffer
1 x 1.25 mL
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date indicated on the outer box label.
On Blue Ice.