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    Cat. No.
    Size
    List Price*
    QTY
    BIO-65027
    2500u
    $108.00
    +
    BIO-65028
    10,000u
    $357.00
    +
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    Description

    RiboSafe RNase Inhibitor is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C.

    Product Highlights

    • Efficient – effectively inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C
    • High-quality - certified free of contaminating DNases, RNases, phosphatases and nickases for improved sample security
    • Robust – active up to 55 oC across a wide range of DTT concentrations and pH
    • Flexible – compatible with all common reverse transcriptases, bacterial polymerases and thermostable polymerases

    Product Description

    Ribonucleases (RNases) are ubiquitous and can be introduced into experiments in many ways: for example co-purification during RNA isolation, carryover from bare hands and pipette tips. This RNase contamination can often go unnoticed. RiboSafe RNase Inhibitor is ideal for RNA-sensitive applications such as RT-qPCR as even a small amount of RNase can be detrimental to the final experimental outcome.

    RiboSafe RNase Inhibitor is a highly efficient inhibitor of a broad spectrum of eukaryotic RNases and shows no inhibition of polymerase or reverse transcriptase activity, so can be used in cDNA synthesis or one-step RT-qPCR reactions.

    RiboSafe RNase Inhibitor is tested for, purity (run on SDS-PAGE gel), presence and absence of endonucleases, nickases and exonucleases, thermostability, inhibition at different pH levels and activity in the presence or absence of DTT. This quality control allows RiboSafe RNase Inhibitor to be used in highly-sensitive techniques such as single-cell RT-PCR, in vitro RNA synthesis and in vitro translation.

    Applications

    • RNA purification
    • cDNA synthesis
    • One-step RT-PCR
    • One-step RT-qPCR
    • In vitro transcription/translation
    • RNA Sequencing
    • RNase protection assays
    • Enzymatic RNA labeling reaction
    Main
    A highly effective, broad-spectrum eukaryotic RNase inhibitor.

    dNTP Guide

    Download the dNTP Guide with detailed product descriptions and performance data to help you choose the best product for your research

    Specification

    Components

    Reagent

    BIO-65027

    BIO-65028

    RiboSafe RNase Inhibitor (40 u/µL)

    62.5 µL (2,500 u)

    250 µL (10,000 u)

    Storage & Stability

    All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.

    Shipping conditions

    Shipped on dry/blue ice.