- Reproducible – consistent results between technical replicates for increased confidence in results
- Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability
- Sensitive – reliable quantification of low abundance targets and scarce samples
- Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
- Fast – delivers reproducible, accurate assay results in as little as 30 minutes
The SensiFAST™ SYBR Hi-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX.
A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR Hi-ROX Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.
The SensiFAST SYBR® Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.
- Gene expression analysis
- DNA / RNA target detection
- miRNA profiling / quantification
- Copy number variation (CNV) analysis
I had a very good experience with the SensiFAST SYBR. It was quick and easy to use, while also providing me with near perfect R-squared values and dissociation curves. Even when compared to other, more expensive, master mixes, I obtained results of equal or better quality.
Mariah Meyer, University of Montana, DBS, US
Instrument CompatibilityPlease refer to the Real-Time PCR Selection Chart to confirm the SensiFAST SYBR® Hi-ROX Kit is compatible with your instrument.
Certification of Analysis (COAs)
200 x 20µl Reactions
500 x 20µl Reactions
2000 x 20µl Reactions
SensiFAST SYBR® Hi-ROX mix (2x)
2 x 1ml
5 x 1ml
4 x 5ml
- BIO-92002: 250 x 20µl Reactions: 2 x 1ml
- BIO-92005: 500 x 20µl Reactions: 5 x 1ml
- BIO-92020: 2000 x 20µl Reactions: 4 x 5ml
Storage & Stability
All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.
SensiFAST SYBR® Lo-ROX Kit is shipped on dry/blue ice
Human - Rosato R.R., et al. Cancer Res. 67: 9490-500 (2007)
Rat - Remund K., et al. Expt. Lung Res. 35: 359-370 (2009)
Drosophila - Brown A.E., et al. PloS ONE 4: e4490 (2009)
Nitrogen-fixing bacteria - Bahlawane C., et al. Mol. Plant-Microbe Inter. 21: 1498-1509 (2008)
Diphtheria bacteria - Jochmann N., et al. Microbiology 155: 1459-1477 (2009)
Green algae - Wobbe L., et al. PNAS 106: 13290-13295 (2009)
Bovine virus - Park S-I., et al. J. Virol. Methods. 159: 64-6 (2009)
For cDNA/DNA templates SensiFAST SYBR® and Probe kits are used. To give you some idea of a few of the types of templates used with these kits:
Stem Cell - Bernardo A.S., et al. Stem Cells 27(2): 341 -351 (2008)
Cow - Baumert A., et al. J. Dairy Research 76(3): 356-364 (2009)
Mouse - Hoyles R.K., et al. Arthritis Rheum. 58(4): 1175-88 (2008)
Quail - De Winter P., et al. British Poultry Science 49(5): 566 – 573 (2008)
Insect - Bass C., et al. Malaria journal 6 111 (2007)
Fish - Miller M.R., et al. J. Nutr. 138(11): 2179-2185 (2008)
Crab - Wilcockson D.C. & Webster S.G. Gen. & Comp. Endo. 156: 113–125 (2008)
Nematode - Murray S.L., et al. Mol. Plant-Microbe Interactions 20(11): 1431–1438 (2007)
Plant - Hecht V., et al. Plant Physiology Preview DOI:10.1104/pp.107.096818 (2007)
Yeast - Kawauchi J., et al. Genes & Dev. 22: 1082-1092 (2008)
E.coli Bacteria - Saeed H.A., et al. Research J. Microbiology 4(4); 173-177 (2009)
Virus - Muscillo M., et al. Water, Air, & Soil Pollution 191: 1-4 (2008)