Ordering
Description
Product Highlights
- Fast – streamlined protocol for consistent recovery of genomic DNA in as little as 20 minutes
- High-performance – extraction of high-purity DNA, ideal for use in all downstream applications
- High-yield – efficient sample lysis and column-binding for high-yield recovery of genomic DNA from limiting amounts of sample
- Flexible – reliable extraction of genomic DNA from a very broad range of human, animal and microbial samples
Product Description
By combining Proteinase K lysis with the speed and convenience of silica membrane purification, ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials.
By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded issue (FFPE), insects, dental swabs, buccal swabs, stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgordferi) and viral DNA (e.g. CMV) from urine.
ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with SensiFAST Real-Time PCR Kits and in end-point PCR with MyTaq DNA Polymerase.
Applications
- qPCR
- End-point PCR
- Southern, dot and slot blotting
- Genotyping
- Restriction digestion
- Next Generation Sequencing
- Bisulfite conversion/methylation analysis
Nucleic Acid Isolation Guide
Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your researchI used the ISOLATE II Genomic DNA Kit to extract genomic DNA from mice tails for genotyping. The kit comes with very clear, user friendly instructions and provides efficient, reliable and cost effective isolation of DNA. Overall a great kit for genomic DNA extraction and downstream analysis.
Wilson Wong, University of Sydney, Camperdown, Australia
Product Selection
Please refer to the ISOLATE II Selection Chart to confirm the applications for which the ISOLATE II Genomic DNA Kit is recommended.Resources
Documents
Certification of Analysis (COAs)
Specification
Components
|
Reagent |
10 Preps |
50 Preps |
250 Preps |
|
ISOLATE II Genomic DNA Spin Columns |
10 |
50 |
250 |
|
Collection Tubes (2 mL) |
20 |
100 |
500 |
|
Lysis Buffer GL |
5 mL |
20 mL |
100 mL |
|
Buffer G3 |
10 mL |
15 mL |
75 mL |
|
Wash Buffer GW1 |
6 mL |
30 mL |
150 mL |
|
Wash Buffer GW2 (concentrate) |
6 mL |
12 mL |
50 mL |
|
Elution Buffer G |
13 mL |
13 mL |
60 mL |
|
Proteinase K (lyophilized) |
6 mg |
30 mg |
2 x 75 mg |
|
Proteinase K Buffer PR |
1.8 mL |
1.8 mL |
8 mL |
|
Bench Protocol Sheet |
1 |
1 |
1 |
Storage & Stability
All components should be stored dry and at room temperature.
When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.
Shipping conditions
Ambient temperature.
FAQs
For the optimal recovery of large RNA/DNA fragments it is necessary to optimize the elution step. To increase the recovery rate it would be helpful to use a larger volume for elution, incubate the column with the elution buffer prior to centrifugation and use repeated elution steps. To avoid excessive dilution it may be helpful to reapply the eluted fraction again. Furthermore it could be helpful for DNA elution to heat the elution buffer to 70°C.
