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  • MyTaq™ DNA Polymerases Review

    21 décembre 2016



    James and Josh from the School Of Biological Sciences at the University of Queensland talk about why they choose to use our MyTaq™ DNA Polymerases.

    To learn more about the MyTaq™ product range, visit https://www.bioline.com/mytaq.html

    iGEM 2015

    11 juin 2015

    It’s that time of year again!!!! We are proud to announce Bioline is supporting iGEM teams in 2015. Our already competitive pricing drops 40% for the young and enthusiastic iGEM researchers in the United Kingdom.

    ‘Sponsorship is very difficult, particularly for small projects such as ours. Bioline are very kindly sponsoring us. A big thank you to them.- York iGEM 2014 Team

    Popular tools used by successful iGEM teams around the world include:

    Get in touch with your local UK Bioline Account Manager to enquire about an iGEM offer pricing plan.

    Then, why not let your fellow iGEM researchers know about this great offer using the sharing links below? Also feel free to Tweet or email us if you have any further questions www.bioline.com/social We look forward to hearing from you soon and we wish all iGEM2015 entrants every success in this year's competition!

    Terms and Conditions
    This offer is valid on any product in our range for any iGEM team in the United Kingdom. No minimum or maximum spend to qualify for the discount. Expires on September 30, 2015. Not valid with any other promo codes or special offers. Please contact us for details of discounts available to iGEM teams in the United States, Germany, France, Singapore and Australia.

    Bioline's Gem of an iGEM Offer is Back for iGEM2014!

    19 juin 2014 No comments

    Bioline is proud once again to offer its support to teams participating in the 2014 round of the annual iGEM competition. Teams supported by Bioline in previous years have won gold medals and advanced to the finals of the iGEM World Championships.

    For a limited time only, all iGEM teams in the United Kingdom can take advantage of up to 40% off already highly-competitive Bioline list prices across our entire product range.

    The Must-Have Bioline Toolbox for iGEM Researchers

    Popular tools used by successful iGEM teams around the world include:

    We used bioline cells but didn’t follow the protocols because the cells were more competent so didn’t need to go through heat shock.Boston iGEM 2012 Team

    We also supply a handy range of antibiotics, SOC medium and a rather nifty quick stick ligase for both cohesive and blunt end cloning.

    All Bioline products are backed by our friendly and helpful UK based technical support staff, should you need it!

    To request full details of our Gem of an iGEM Offer pricing plan, please contact your local Bioline UK account manager using our Rep Finder tool. Then, why not let your fellow iGem researchers know about this great offer using the sharing links below. Also feel free to Tweet or email us if you have any further questions

    We look forward to hearing from you soon and we wish all iGEM2014 entrants every success in this year's competition!

    PS: You can also read about Bioline’s Synthetic Biology industry partnership with Liverpool Life Sciences UTC, the first school in the United Kingdom specialising in Science and Health Care for 14 to 19 year olds.

    Terms and Conditions

    This offer is valid on any product in our range for any iGEM team in the United Kingdom. No minimum or maximum spend to qualify for the discount. Expires on October 31, 2014. Not valid with any other promo codes or special offers. Please contact us for details of discounts available to iGEM teams in the United States, Germany, France, Singapore and Australia. Bioline products are also available through our carefully selected distributor partners.

    Bioline Scholar - A CRISPR way of gene editing

    4 décembre 2013 No comments

    An alternative way for RNAi libraries to modify the mammalian genome on a large-scale may lie in the use of genome editing technologies such as the use of targeted endonucleases. Such a technique includes Zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered, regularly-interspaced, short palindromic repeats/CRISPR-associated endonuclease cas9 (CRISPR/Cas9). CRISPR is a Cas9 endonuclease–based method for sequence-specific genome modification. It can be used to facilitate efficient genome engineering in eukaryotic cells by simply specifying a 20-nt targeting sequence within its guide RNA.

    These technologies have proven highly useful for gene editing genomes of various species and the speed at which CRISPR techniques have been exploited has been incredibly rapid.

    In January 2013 researchers at MIT, the Broad Institute, and Rockerfeller University first reported the new technique based on the CRISPR/Cas9 system, for precisely altering the genomes of living cells through gene addition or deletion. Researchers say the technology could offer an easy-to-use and more cost-effective way to engineer organisms that produce biofuels; to design animal models to study human disease; or to develop new therapies, among other potential applications.

    Bioline reagents have been used to augment the workflow in these exciting gene editing technologies to elucidate CRISPR systems, including our SensiFAST™ range of Real-Time PCR Kits, as well as our performance PCR polymerases.

    SensiFAST™ No-ROX One-step Kit

    In the first examples of genes that mediate the inhibition of a CRISPR/Cas system, researchers from the University of Toronto identified five distinct ‘anti-CRISPR’ genes in the genomes of bacteriophages infecting Pseudomonas aeruginosa.

    Bondy-Denomy, J et al., (2012). Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system. Nature 493, 429-432.

    MyTaq™ Red Mix

    Researchers from Minnesota University reported precise, high-frequency editing of 15 genes in pig, goat, and cattle genomes using CRISPR/Cas and TALENs technology. RFLP analysis of colonies were analysed by PCR using MyTaq Red Mix.

    Wenfang, T.W. et al., (2013). Efficient nonmeiotic allele introgression in livestock using custom endonucleases. PNAS 110(41), 16526-16531.

    To learn more about performance PCR reagents from Bioline, just visit www.bioline.com

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