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Ordering

Cat. No.
Size
BIO-33066
50-100 Lanes

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Description

A broad range prestained protein marker containing 9 purified recombinant proteins with molecular weights ranging from 10 kDa to 190 kDa, for easy size determination of proteins in SDS-PAGE and transfer to a membrane.

Product Highlights

  • Easy size determination - 9 prestained bands
  • Broad range - 10 to 190 kDa
  • Multi-colored - for easy identification and orientation
  • Easy-to-use - giving clear and sharp band resolution

Product Description

HyperPAGE is a broad range protein marker containing 9 purified prestained recombinant proteins with molecular weights ranging from 10 kDa to 190 kDa, ideal for molecular weight determination for a wide range of expressed proteins. The proteins are prestained and resolve into 4 blue and 5 pink bands with uniform intensity, for easy visualization and orientation.

HyperPAGE is suitable to monitor the efficiency of electrophoretic migration in SDS-PAGE and the transfer of proteins from gel to membrane in western blotting without the addition of dye. The HyperPAGE proteins have been labeled with fluorescent dyes and are supplied lyophilized, containing the buffer salts and detergent found in the typical SDS-PAGE buffer system.

Applications

  • Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.
  • Monitoring of protein transfer onto membranes during western blotting.
  • Sizing of proteins on SDS-polyacrylamide gels and western blots.
Main

Product Selection

Please refer to the HyperLadder Selection Chart to confirm the HyperPAGE is the most suitable kit for your application.


Specification

Components

Reagent

50-100 lanes

HyperPAGE Prestained Protein Marker

500 µL

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Once reconstituted with water the product is stable for three months at -20°C.

Shipping conditions

Freeze-dried and shipped on blue ice.



FAQs

Prestained proteins may display a different mobility in different SDS-PAGE buffer systems. Each buffer system has a slightly different pH which affects the charge of a protein and its binding capacity for SDS. However, HyperPAGE is suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. For accurate sizing, the use of unstained markers is recommended.