To view our range of antigens and antibodies for immunoassay development please visit our partner site meridianlifescience.com

Total RNA

The ISOLATE II range includes kits designed for the isolation and purification of total RNA from a wide variety of samples, including samples that are sometimes difficult to process. The kits are supplied with filters (shredders) and DNase where required, to enhance sample homogenization and remove genomic DNA. The ISOLATE II range does not incorporate organic solvents, thereby improving safety and avoiding bias in recovery between different RNA species.

The ISOLATE II RNA Kits have been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST™ cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II RNA Mini Kit can be used to purify samples for PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Meridian PCR portfolio, including MyTaq™ DNA Polymerase.

  • Fast – simple extraction of high purity total RNA ideal for use in all applications
  • High-performance – recovery of consistently high quality RNA from a wide variety of samples
  • Efficient – optimized lysis conditions and column matrix for maximum recovery of high purity RNA
  • Convenient – includes all necessary components, including filters (shredders) and DNase I
  • Safe - no hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl or alcohol precipitation

ISOLATE II Guide

Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your research

ISOLATE II Selection Chart

Select the best reagent for your research based on your sample requirements

Working with RNA Hints and Tips

Hints and tips to help you maintain an RNase-free environment

“We have been utilizing ISOLATE II RNA Mini Kit from Meridian and have found that the RNA yield, concentrations and purity have been consistently good. From 40,000 liver cancer (mouse and human) cells we are able to get 200-600 ng/ul yield of RNA.”

Aparna Jayachandran, GMRF-UQ, Brisbane, Australia

Genomic DNA

The ISOLATE II portfolio incorporates kits for the rapid purification of genomic DNA from a broad range of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded tissue (FFPE), stool viruses, bacterial DNA, biological fluids or clinical specimens, bacterial DNA and viral DNA from urine, insects, dental swabs and buccal swabs.

All ISOLATE II Kits for genomic DNA purification employ highly-efficient column-based without the need for hazardous reagents such as phenol, thereby improving safety and circumventing any bias in recovery of different DNA species. The ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR alongside any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.

  • Fast – streamlined protocols for consistent recovery of genomic DNA
  • High-performance – extraction of high-purity DNA, ideal for use in all downstream applications
  • High-yield – efficient sample lysis and column-binding for high-yield recovery of genomic DNA from even very limiting amounts of sample
  • Flexible – reliable extraction of genomic DNA from a very broad range of human samples

Plasmid DNA

The ISOLATE II portfolio includes a kit for simple, rapid, efficient recovery of plasmid DNA from even low bacterial culture volumes, without the need for hazardous reagents such as phenol. Separate protocols are provided for the column-based purification of high-copy plasmids, low-copy plasmid, P1 constructs and cosmid DNA from E. coli as well as the purification of plasmids from gram-positive bacteria.

PCR Clean Up

Rapid, simple, efficient membrane-based purification of DNA from PCR reactions and from TAE and TBE agarose gels, as well as PCR reactions, without the need for hazardous reagents or ethanol precipitation. The ISOLATE II PCR and Gel Kit is ideal for purifying DNA for use in transformations, cloning, sequencing and restriction analysis.

Column-free Purification

TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials. By combining thiocyanate compounds with phenol, TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population.


Products

TRIsure™

Reviews

"I recently used the ISOLATE II RNA Mini kit. I had some cultured cells which are ..."

"We used the Isolate II RNA Micro Kit, SensiFAST cDNA synthesis kit and ..."

"We have been utilizing ISOLATE II RNA Mini Kit from Meridian and have found ..."

"I have used this kit to extract genomic DNA from mice tails (approx. 0.6 cm) for ..."

"The ISOLATE II Genomic DNA Kit is extremely sensitive. It produced ..."