The ISOLATE II range includes kits designed for the isolation and purification of total RNA from a wide variety of samples, including samples that are sometimes difficult to process. The kits are supplied with filters (shredders) and DNase where required, to enhance sample homogenization and remove genomic DNA. The ISOLATE II range does not incorporate organic solvents, thereby improving safety and avoiding bias in recovery between different RNA species.
Hints and tips to help you maintain an RNase-free environment
“We have been utilizing ISOLATE II RNA Mini Kit from Meridian and have found that the RNA yield, concentrations and purity have been consistently good. From 40,000 liver cancer (mouse and human) cells we are able to get 200-600 ng/ul yield of RNA.”
Aparna Jayachandran, GMRF-UQ, Brisbane, Australia
The ISOLATE II portfolio incorporates kits for the rapid purification of genomic DNA from a broad range of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded tissue (FFPE), stool viruses, bacterial DNA, biological fluids or clinical specimens, bacterial DNA and viral DNA from urine, insects, dental swabs and buccal swabs.
All ISOLATE II Kits for genomic DNA purification employ highly-efficient column-based without the need for hazardous reagents such as phenol, thereby improving safety and circumventing any bias in recovery of different DNA species. The ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR alongside any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase.
Fast – streamlined protocols for consistent recovery of genomic DNA
High-performance – extraction of high-purity DNA, ideal for use in all downstream applications
High-yield – efficient sample lysis and column-binding for high-yield recovery of genomic DNA from even very limiting amounts of sample
Flexible – reliable extraction of genomic DNA from a very broad range of human samples
The ISOLATE II portfolio includes a kit for simple, rapid, efficient recovery of plasmid DNA from even low bacterial culture volumes, without the need for hazardous reagents such as phenol. Separate protocols are provided for the column-based purification of high-copy plasmids, low-copy plasmid, P1 constructs and cosmid DNA from E. coli as well as the purification of plasmids from gram-positive bacteria.
Rapid, simple, efficient membrane-based purification of DNA from PCR reactions and from TAE and TBE agarose gels, as well as PCR reactions, without the need for hazardous reagents or ethanol precipitation. The ISOLATE II PCR and Gel Kit is ideal for purifying DNA for use in transformations, cloning, sequencing and restriction analysis.
TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials. By combining thiocyanate compounds with phenol, TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population.