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Ordering

Cat. No.
Size
BIO-52082
50 Preps

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Description

Highly efficient purification of host-cell and microbial DNA by combining fast lysis of the starting material in the presence of guanidinium thiocyanate with the speed and purity of silica-membrane column purification.

Product Highlights

  • Fast – streamlined protocol for extraction of host cell and microbial DNA in as little as 15 minutes
  • High-performance – recovery of consistently high-quality DNA, ideal for use in all downstream applications
  • Efficient – rapid and effective lysis of fecal samples by bead beating for excellent recovery of DNA
  • Flexible – reliable recovery of host and microbial DNA from human and a broad range of animal species

Product Description

The ISOLATE II Fecal DNA Kit provides a simple, efficient column-based method for the isolation of DNA from a wide variety of fecal samples, without the need for hazardous reagents such as phenol.

By combining the stringency of guanidinium-thiocyanate lysis and high-density bashing beads and the speed and ease-of-use of silica-membrane purification to remove humic acids/polyphenols, the ISOLATE II Fecal DNA Kit provides a fast method for the purification of high-quality bacterial/protist DNA from a variety of fecal samples including humans, birds, rats, mice, rabbits, cattle, etc. as well as host DNA.

The ISOLATE II Fecal DNA Kit has been designed to purify samples ideal for use in end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase and in qPCR using the the SensiFAST Real-Time PCR Kits.

Applications

  • End-point PCR
  • qPCR
  • Southern, dot and slot blotting
  • Genotyping
  • Next Generation Sequencing
  • Bisulfite conversion/methylation analysis
Main

Nucleic Acid Isolation Guide

Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your research

Product Selection

Please refer to the ISOLATE II Selection Chart to confirm the applications for which the ISOLATE II Fecal DNA Kit is recommended.


Specification

Components

Reagent

50 Preps

100 Preps

Lysis Buffer

1 x 40 mL

2 x 40 mL

DNA Pre-Wash Buffer

1 x 15 mL

2 x 15 mL

Fecal DNA Wash Buffer

1 x 50 mL

2 x 50 mL

Fecal DNA Binding Buffer

1 x  100 mL

2 x 100 mL

DNA Elution Buffer

1 x 10 mL

2 x 10 mL

Fecal Prep Solution

1 x 30 mL

2 x 30 mL

Bashing Beads Lysis Tubes

1 x 50

2 x 50

Spin II A Filters

1 x 50

2 x 50

Spin II B Filters

1 x 50

2 x 50

Spin Columns

1 x 50

2 x 50

Collection Tubes

200

2 x 200

Bench Protocol Sheet

1

1

Storage & Stability

All components should be stored dry and at room temperature. When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

Ambient temperature.



Resources

Reviews

"Aim: To compare the sensitivity of two commercial faecal DNA extraction kits ...
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FAQs

The columns supplied with the ISOLATE II kits may appear similar, but each type has been optimized to work within the buffer system supplied with the corresponding kit. The swapping of columns (or buffers) between kits may lead to no recovery of nucleic acid whatsoever, or at the very least severely impaired purification.

Interruption of the DNA/RNA extraction process is possible after the sample lysis only. It is possible to homogenize and lyse the samples and to store them in the freezer until use for RNA extraction. RNA clean up with a column cannot be interrupted and we recommend to avoid delays during the column purification process. If a delay is unavoidable the columns should be stored on ice.

The elution buffer is ideal for applications such as restriction enzyme digestion, sequencing and PCR. It is possible to use DNase-free water for elution, but you should expect a slightly lower yield.

Bacterial spores are effectively lysed with the bead lysis step. The use of high-speed disruptor devices and an optimization of sample incubation time are recommended.