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The ISOLATE II Fecal DNA Kit provides a simple, efficient column-based method for the isolation of DNA from a wide variety of fecal samples, without the need for hazardous reagents such as phenol.
By combining the stringency of guanidinium-thiocyanate lysis and high-density bashing beads and the speed and ease-of-use of silica-membrane purification to remove humic acids/polyphenols, the ISOLATE II Fecal DNA Kit provides a fast method for the purification of high-quality bacterial/protist DNA from a variety of fecal samples including humans, birds, rats, mice, rabbits, cattle, etc. as well as host DNA.
The ISOLATE II Fecal DNA Kit has been designed to purify samples ideal for use in end-point PCR alongside any enzyme from the Meridian PCR portfolio, including MyTaq DNA Polymerase and in qPCR using the the SensiFAST Real-Time PCR Kits.
PCR amplification of DNA extracted from mouse feces
Genomic DNA was extracted from increasing amounts of mouse feces (6 mg, 12.5 mg, 29 mg, 53.5 mg, 104 mg and 139 mg (lanes 1-6 respectively)) and mouse tail (control) using ISOLATE Fecal DNA Kit. PCR was performed using two primer sets for the Rn 18s gene (400 bp and 1 kb). The consistent yield of PCR product from all of the samples indicates efficient removal of PCR inhibitors, even with larger volumes of starting material.
Consistency of extraction of DNA from mouse feces
Genomic DNA was extracted from 2 uL of mouse fecal matter from different sources and compared to mouse tail DNA (control), using ISOLATE Fecal DNA Kit. PCR was performed using a primer sets for the Rn 18s gene (1.8 kb). The results illustrate the consistency of the yield of DNA from all of the fecal samples.
Reagent |
50 Preps |
100 Preps |
Lysis Buffer |
1 x 40 mL |
2 x 40 mL |
DNA Pre-Wash Buffer |
1 x 15 mL |
2 x 15 mL |
Fecal DNA Wash Buffer |
1 x 50 mL |
2 x 50 mL |
Fecal DNA Binding Buffer |
1 x 100 mL |
2 x 100 mL |
DNA Elution Buffer |
1 x 10 mL |
2 x 10 mL |
Fecal Prep Solution |
1 x 30 mL |
2 x 30 mL |
Bashing Beads Lysis Tubes |
1 x 50 |
2 x 50 |
Spin II A Filters |
1 x 50 |
2 x 50 |
Spin II B Filters |
1 x 50 |
2 x 50 |
Spin Columns |
1 x 50 |
2 x 50 |
Collection Tubes |
200 |
2 x 200 |
Bench Protocol Sheet |
1 |
1 |
All components should be stored dry and at room temperature. When stored under the recommended conditions and handled correctly, full activity of reagents is retained until the expiry date indicated on the outer box label.
Ambient temperature.