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Description
Reaction clean-up, as well as a single- or double-sided size selection of libraries following the fragmentation, ligation and PCR steps in the next-generation sequencing (NGS) workflow.
Product Highlights
Precise - consistent bead size for highly-reproducible selection of the user-defined fragment range
Efficient - excellent recovery of fragments greater than 100 bp and efficient removal of all contaminants
Flexible - highly effective clean-up from all types of fragmentation, ligation and PCR reactions
Fast - eliminates centrifugation or filtration steps for fast manual processing and straightforward integration with automated liquid handling platforms
Robust - bead composition specifically developed to withstand the rigours of the NGS workflow
Product Description
JetSeq™ Clean is a NGS library preparation clean-up system based on paramagnetic bead technology, designed for efficient purification of nucleic acid fragments in next-generation sequencing workflow. JetSeq Clean provides maximum flexibility allowing for left-, right- or double-sided size selection.
JetSeq Clean removes, salts, primers, primer-dimers and dNTPs, while library fragments are selectively bound to the magnetic particles based on their size. Purified library fragments are eluted from the magnetic particles using water or a low salt buffer and can be used directly for all downstream NGS applications. The protocol can be performed manually, or adapted to liquid handling workstations (e.g. Agilent, Beckman, Caliper, Eppendorf, Hamilton, PerkinElmer and Tecan) using 96- or 384-well formats.
Applications
NGS
Sanger Sequencing
PCR
Nucleic acid clean up
Fragment analysis
Removal of PCR contaminants by JetSeq Clean bead purification.
Electropherogram and virtual gel view showing removal of unincorporated adapters and PCR primers (arrows) after using the JetSeq Clean
Size distribution after right-side size selection
For right-side size selection, the fragmented sample is mixed with JetSeq Clean to a range of sample to bead ratios to recover fragments below an upper size limit. The peak electropherogram view results illustrate the precision of JetSeq Clean in selection of a specific, user-defined range of smaller fragments for sequencing.
Size distribution after right-side size selection
For right-side size selection, the virtual gel view results illustrate the precision of JetSeq Clean in selection of a specific, user-defined range of smaller fragments for sequencing.
Size distribution after left-side size selection
For left-side size selection, the fragmented sample is mixed with JetSeq Clean to a range of sample to bead ratios to recover fragments above a lower size limit. The peak electropherogram view results illustrate the precision of JetSeq Clean in selection of a specific, user-defined range of larger fragments for sequencing.
Size distribution after left-side size selection
For left-side size selection, the virtual gel view results illustrate the precision of JetSeq Clean in selection of a specific, user-defined range of larger fragments for sequencing.
Size distribution after double-side size selection
Double-side size selection, supernatant from a right-side selection step, is used for left side size selection process to exclude the largest and smallest library fragments respectively. The results illustrate that adjustment of the size selection parameters allows JetSeq Clean to recover a highly specific range of library fragments and remove unwanted reaction components prior to sequencing.
Instrument Compatibility with liquid handling workstations from suppliers such as Agilent, Beckman, Caliper, Eppendorf, Hamilton, PerkinElmer and Tecan, using 96- or 384-well formats.
