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*link will take you to our exclusive distribution partner site
The generation of high-quality PCR-free next-generation sequencing (NGS) libraries, suitable for sequencing on Illumina® instruments.
The success of next-generation sequencing is dependent upon the precise and accurate processing of the input DNA. This requires high-quality library preparation using a coordinated series of standard molecular biology reactions whilst maintaining high yields during the intermediate purification steps.
The JetSeq™ ER and Ligation Kit is designed to generate high-quality PCR-free, next-generation sequencing (NGS) libraries making it ideal for whole-genome sequencing applications on Illumina MiniSeq™, MiSeq™, NextSeq™ or HiSeq™ instruments. The kit contains all of the enzymes and buffers necessary for end-repair, A-tailing and ligation in convenient master mix formulations. It offers a fast, streamlined workflow and flexibility where users are free to use adapters of their choice.
By combining end-repair and A-tailing in one unique step, the JetSeq ER and Ligation Kit is able to reduce total NGS library preparation time and minimize the variability caused by additional handling, as well as the risk of contamination and material loss.
DNA Library Preparation workflow
The JetSeq NGS Library Preparation Kit incorporates fewer steps. The simpler, shorter protocols reduce both hands-on time and the total time required for preparation of library DNA.
Library conversion rates
The libraries were prepared with from 10 ng or 100 ng of human genomic DNA using the manufacturers’ recommendations. The library conversion rates were normalized to JetSeq Kit, demonstrating that JetSeq has a higher library conversion rate than Suppliers I and N.
Reagent |
|
End-Repair Buffer, 5x |
960 μL |
ER Enzyme Mix |
576 μL |
Ligation Buffer, 5x |
288 μL |
Ligase |
192 μL |
Nuclease-Free Water |
2 x 1.8 mL |
On Dry Ice or Blue Ice.