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*link will take you to our exclusive distribution partner site
The generation of high-quality next-generation sequencing (NGS) libraries, includes a PCR step for library amplification, where starting material is limited. Suitable for sequencing on Illumina® instruments.
The success of next-generation sequencing is dependent upon the precise and accurate processing of the input DNA. This requires high-quality library preparation using a coordinated series of standard molecular biology reactions whilst maintaining high yields during the intermediate purification steps.
The JetSeq™ Flex DNA Library Preparation Kit is designed to generate high-quality next-generation sequencing (NGS) libraries, even with more limited amounts of starting material and is suitable for sequencing on Illumina MiniSeq™, MiSeq™, NextSeq™ or HiSeq™ instruments. The kit contains all of the enzymes and buffers necessary for end-repair, A-tailing, ligation and PCR amplification in convenient master mix formulations. It offers a fast, streamlined workflow and flexibility where users are free to adapters of their choice.
By combining end-repair and A-tailing in one unique step, the JetSeq Flex DNA Library Preparation Kit is able to reduce total NGS library preparation time and minimize the variability caused by additional handling, as well as the risk of contamination and material lose. A high quality DNA polymerase is also supplied to selectively enrich library fragments carrying the adapter sequences and to amplify the amount of DNA prior to sequencing.
DNA Library Preparation workflow
The JetSeq NGS Library Preparation Kit incorporates fewer steps. The simpler, shorter protocols reduce both hands-on time and the total time required for preparation of library DNA.
Library conversion rates
The libraries were prepared with from 10 ng or 100 ng of human genomic DNA using the manufacturers’ recommendations. The library conversion rates were normalized to JetSeq Kit, demonstrating that JetSeq has a higher library conversion rate higher to the competitors.
JetSeq Flex DNA Library Preparation Kit is compatible with the Illumina MiniSeq, MiSeq, NextSeq and HiSeq instruments.
Reagent |
|
End-Repair Buffer, 5x |
960 μL |
ER Enzyme Mix |
576 μL |
Ligation Buffer, 5x |
288 μL |
Ligase |
192 μL |
PCR Buffer, 10x |
480 μL |
DNA Polymerase |
192 μL |
Nuclease-Free Water |
4 x 1.8 mL |
On Dry Ice or Blue Ice.