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Description
The generation of high-quality next-generation sequencing (NGS) libraries, includes a PCR step for library amplification, where starting material is limited. Suitable for sequencing on Illumina® instruments.
Product Highlights
Low input - end-repair, A-tailing and ligation combined in the same tube, thereby eliminating cleanup steps and improving sample yield
Improved confidence - simpler protocol with fewer steps for reduced risk of sample loss and offering greater peace-of-mind
Increased speed - fast library preparation for reduced time to results and increased sample
throughput
Highly efficient - reaction buffer pre-optimized to provide maximum reaction efficiency and highest conversion rates
High-yield - PCR polymerase and buffer, developed specifically for library preparation, giving maximum yield of sequence-ready library DNA
Improved quality - optimized, high quality reagents result in reliable library preparation from even very challenging samples, providing maximum coverage
Flexibility – available as a PCR and a PCR-free kit, for use with Illumina adapters and indexes
Product Description
The success of next-generation sequencing is dependent upon the precise and accurate processing of the input DNA. This requires high-quality library preparation using a coordinated series of standard molecular biology reactions whilst maintaining high yields during the intermediate purification steps.
The JetSeq™ Flex DNA Library Preparation Kit is designed to generate high-quality next-generation sequencing (NGS) libraries, even with more limited amounts of starting material and is suitable for sequencing on Illumina MiniSeq™, MiSeq™, NextSeq™ or HiSeq™ instruments. The kit contains all of the enzymes and buffers necessary for end-repair, A-tailing, ligation and PCR amplification in convenient master mix formulations. It offers a fast, streamlined workflow and flexibility where users are free to adapters of their choice.
By combining end-repair and A-tailing in one unique step, the JetSeq Flex DNA Library Preparation Kit is able to reduce total NGS library preparation time and minimize the variability caused by additional handling, as well as the risk of contamination and material lose. A high quality DNA polymerase is also supplied to selectively enrich library fragments carrying the adapter sequences and to amplify the amount of DNA prior to sequencing.
DNA Library Preparation workflow
The JetSeq NGS Library Preparation Kit incorporates fewer steps. The simpler, shorter protocols reduce both hands-on time and the total time required for preparation of library DNA.
Library conversion rates
The libraries were prepared with from 10 ng or 100 ng of human genomic DNA using the manufacturers’ recommendations. The library conversion rates were normalized to JetSeq Kit, demonstrating that JetSeq has a higher library conversion rate higher to the competitors.
