JetSeq™ Lyophilized NGS Library Prep Kit

Figure 1 Library quality

The DNA from the prepared libraries were A) run on a Bioanalyzer®, the red traces representing libraries made with the lyophilized library preparation kit and the blue traces libraries made with all liquid reagents. The results illustrate that both lyophilized and liquid formulations generated libraries are of similar quality, ready for sequencing.

Figure 2 Library yield

The DNA from the prepared libraries were quantified using a qPCR-based NGS Library Quantification Kit, with P5 and P7 primers, ®, the red representing libraries made with the lyophilized library preparation kit and the blue, libraries made with all liquid reagents. The results illustrate that both lyophilized and liquid formulations generated libraries are of similar yield, demonstrating that the lyophilized reagents match the performance of liquid reagents, producing good-yield libraries ready for sequencing.

Figure 3 Sequencing data quality

A) The number of reads before trimming, normalized to the number of reads produced by libraries prepared with liquid reagents and expressed as a percentage. The results illustrate that that both lyophilized and liquid formulations generated libraries create comparable numbers of reads.