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Description
MyTaq™ Plant-PCR Kit offers fast, highly-specific, direct PCR from a wide range of plant leaf samples. The novel buffer system circumvents the need for additional PCR stabilizers and traditional purification steps, thereby avoiding a complex extraction process.
Product Highlights
Fast - eliminate complex, slow and costly DNA extraction steps, thereby reducing time to results
Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving yield of even the most challenging targets
Robust- specially developed to overcome common plant-derived PCR inhibitors such as polyphenolics and polysaccharides for highest PCR success rates and improved sensitivity
Versatile - perfect for a wide range of plant species, avoiding the need for further optimization, thereby minimizing setup time and reducing cost
Simple - ideal for fast genotyping in plant genetic studies, mutation detection, confirming transgenic plant and knockout analysis
Product Description
MyTaq™ Plant PCR Kit is recommended for fast, specific and direct PCR from a wide range of plant leaf samples. MyTaq Plant-PCR Kit incorporates MyTaq HS DNA Polymerase, a next-generation hot-start polymerase that delivers highly-specific PCR amplification. Furthermore, MyTaq HS has an increased affinity for template DNA, giving a high PCR product yield for most challenging templates. MyTaq HS DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases meaning it performs reliably even in the presence of PCR inhibitors.
MyTaq Plant-PCR Kit includes novel buffer system that replaces the need for complicated extraction or purification steps, including freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction or column DNA purification. The advanced formulation of MyTaq Plant-PCR Kit also allows fast cycling conditions to be used, without compromising PCR specificity and yield.
MyTaq Plant-PCR Kit has been developed to tolerate the PCR inhibitors typically present in plant samples, including polyphenolics and polysaccharides, thereby delivering significantly improved assay sensitivity and reproducibility. MyTaq Plant-PCR Kit increases amplification success rates from different plant types and its speed and high specificity makes it ideal for high-throughput genotyping assays.
Bioline R&D has obtained successful results when using the MyTaq Plant-PCR Kit to conduct direct PCR from a very broad range of species. These include tomato, rice, sugarcane, maize, potato, soya, wheat, barley, cashew, oak, dwarf umbrella tree, Australian laurel, red-fruit saw-sedge, blueberry lily, she-oaks, coastal rosemary, burrawang, eucalyptus/gum tree, seaweed/crayweed, coastal banksia, bottle brush and honey gem. For more information, please inquire.
As part of our test and review campaign, many of our customers have shared their positive experiences of the MyTaq Plant-PCR Kit. Our customers have also reported success for direct PCR from wheat, rosemary, cotton, tobacco, citrus, kangkong, mousear cress (Arabidopsis), cranberry, primrose, blueberry, American bellflower and several grass and fungus species.
Applications
Genotyping for plant genetic study
Mutation detection
Transgenic detection
Knockout analysis
Fig. 1 Increasing concentration of inhibitors in a reaction
Amplification of increasing numbers of rice leaf punches. Increasing the number of leaf punches also increases the concentration of PCR inhibitors in the reaction. The thermal cycling conditions were set according to the manufacturers recommendations. The results show that MyTaq Plant-PCR exhibits improved tolerance to inhibitors, without compromising the PCR efficiency.
Fig. 2 Sensitivity of amplification from different plant samples
Amplification of 0.5 kb fragments directly from leaf punches (1.2 mm diameter) from sugarcane, rice, tomato and maize. 50 μL PCR reactions were set up and run according to the manufacturers recommendations. A two-fold serial dilution of the PCR product was run on an agarose gel to illustrate the higher yield and therefore higher sensitivity of the MyTaq Plant-PCR over other suppliers.
MyTaq™ Plant-PCR Kit Workflow
Dr. Steve Hawkins delivers a simple protocol for Direct Plant PCR using MyTaq™ Plant-PCR Kit.
Introduction to MyTaq
Overview, features and benefits of the MyTaq product family
We tested the mytaq plant direct PCR kit in our Arabidopsis genotyping workflow. It saved us a ton of time by cutting out the tedious isolation of genomic DNA from leaves. We got consistent results using both the direct leaf protocol and the difficult samples protocol using SDS. We found that lysed leaf samples in SDS could be stored at -20°C and reused multiple times over a several weeks with no discernible loss of quality. We also were able to use the direct plant kit to 'rescue' genomic DNA resulting from poor quality isolations that would not amplify with other polymerases.
Mytaq direct plant PCR kit performed better than the competing product we tried. While both kits provided comparable amplification in a 50uL reaction, only the mytaq direct plant PCR kit was able to amplify directly from leaf tissue in a 25uL reaction.
Benson Lindsey, The Ohio State University, Columbus, USA
MyTaq Plant-PCR Kit can be stored at -20°C. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date printed on the outer box label.
"We tested MyTaq Plant Direct PCR kit in our routine Arabidopsis thaliana ...
"
Dr Monika Murcha, School of Molecular Sciences, The University of Western Australia,Australia
"We tested the mytaq plant direct PCR kit in our Arabidopsis genotyping ...
"
Benson Lindsey, The Ohio State University, Columbus, USA
We tested the mytaq plant direct PCR kit in our Arabidopsis genotyping workflow. It saved us a ton of time by cutting out the tedious isolation of genomic DNA from leaves. We got consistent results using both the direct leaf protocol and the difficult samples protocol using SDS. We found that lysed leaf samples in SDS could be stored at -20C and reused multiple times over a several weeks with no discernible loss of quality. We also were able to use the direct plant kit to 'rescue' genomic DNA resulting from poor quality isolations that would not amplify with other polymerases.
Mytaq direct plant PCR kit performed better than the competing product we tried. While both kits provided comparable amplification in a 50uL reaction, only the mytaq direct plant PCR kit was able to amplify directly from leaf tissue in a 25uL reaction.
Benson Lindsey, The Ohio State University, Columbus, USA
We tested MyTaq Plant Direct PCR kit in our routine Arabidopsis thaliana genotyping experiments using genomic DNA extracted from “homemade” methods. We have been getting 100% efficiency with all samples resulting in abundant PCR product. We were particularly pleased with the kits performance on previously known “difficult” plant samples from various mutants. We could not get product even when using commercial high purity genomic DNA kits, with MyTaq we got the products even using crude extracts. We have also found the kit to perform well for screening bacterial colonies. We routinely now use this kit for both methods in the lab.
Dr Monika Murcha, School of Molecular Sciences, The University of Western Australia,Australia
Reviews
"We tested MyTaq Plant Direct PCR kit in our routine Arabidopsis thaliana ...
"
Dr Monika Murcha, School of Molecular Sciences, The University of Western Australia,Australia
"We tested the mytaq plant direct PCR kit in our Arabidopsis genotyping ...
"
Benson Lindsey, The Ohio State University, Columbus, USA
We tested the mytaq plant direct PCR kit in our Arabidopsis genotyping workflow. It saved us a ton of time by cutting out the tedious isolation of genomic DNA from leaves. We got consistent results using both the direct leaf protocol and the difficult samples protocol using SDS. We found that lysed leaf samples in SDS could be stored at -20C and reused multiple times over a several weeks with no discernible loss of quality. We also were able to use the direct plant kit to 'rescue' genomic DNA resulting from poor quality isolations that would not amplify with other polymerases.
Mytaq direct plant PCR kit performed better than the competing product we tried. While both kits provided comparable amplification in a 50uL reaction, only the mytaq direct plant PCR kit was able to amplify directly from leaf tissue in a 25uL reaction.
Benson Lindsey, The Ohio State University, Columbus, USA
We tested MyTaq Plant Direct PCR kit in our routine Arabidopsis thaliana genotyping experiments using genomic DNA extracted from “homemade” methods. We have been getting 100% efficiency with all samples resulting in abundant PCR product. We were particularly pleased with the kits performance on previously known “difficult” plant samples from various mutants. We could not get product even when using commercial high purity genomic DNA kits, with MyTaq we got the products even using crude extracts. We have also found the kit to perform well for screening bacterial colonies. We routinely now use this kit for both methods in the lab.
Dr Monika Murcha, School of Molecular Sciences, The University of Western Australia,Australia
FAQs
"We tested MyTaq Plant Direct PCR kit in our routine Arabidopsis thaliana ..."
Dr Monika Murcha, School of Molecular Sciences, The University of Western Australia,Australia
"We tested the mytaq plant direct PCR kit in our Arabidopsis genotyping ..."
Benson Lindsey, The Ohio State University, Columbus, USA
We tested the mytaq plant direct PCR kit in our Arabidopsis genotyping workflow. It saved us a ton of time by cutting out the tedious isolation of genomic DNA from leaves. We got consistent results using both the direct leaf protocol and the difficult samples protocol using SDS. We found that lysed leaf samples in SDS could be stored at -20C and reused multiple times over a several weeks with no discernible loss of quality. We also were able to use the direct plant kit to 'rescue' genomic DNA resulting from poor quality isolations that would not amplify with other polymerases.
Mytaq direct plant PCR kit performed better than the competing product we tried. While both kits provided comparable amplification in a 50uL reaction, only the mytaq direct plant PCR kit was able to amplify directly from leaf tissue in a 25uL reaction.
Benson Lindsey, The Ohio State University, Columbus, USA
We tested MyTaq Plant Direct PCR kit in our routine Arabidopsis thaliana genotyping experiments using genomic DNA extracted from “homemade” methods. We have been getting 100% efficiency with all samples resulting in abundant PCR product. We were particularly pleased with the kits performance on previously known “difficult” plant samples from various mutants. We could not get product even when using commercial high purity genomic DNA kits, with MyTaq we got the products even using crude extracts. We have also found the kit to perform well for screening bacterial colonies. We routinely now use this kit for both methods in the lab.
Dr Monika Murcha, School of Molecular Sciences, The University of Western Australia,Australia
