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Ordering

Cat. No.
Size
BIO-37084
5ml
BIO-37085
5 x 5ml

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Description

Proteinase K is a highly active serine protease with broad cleavage specificity on native and denatured proteins. Proteinase K is widely used in the purification of native RNA and DNA from tissues or cell lines and many other applications.

Product Highlights

  • High activity - broad-spectrum serine protease
  • Active under denaturing conditions - useful for a variety of applications
  • Stable - at high temperatures
  • Molecular biology grade - useful for general digestion of proteins
  • Proteinase K is also available in powder form

Product Description


Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is especially suitable for isolating PCR and RT-PCR templates.

The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and at elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/mL for protein removal and enzyme inactivation and up to 2 mg/mL for tissue treatment.

Proteinase K products are free of detectable DNase and RNase.

Applications

  • Inactivation of RNases/DNases during nucleic acid extraction
  • Protein modification
  • General protein digestion
  • Determination of enzyme localization
  • Remove nucleases for in situ hybridization
  • Improving cloning efficiency of PCR products

Specification

Components

Reagent

BIO-37084

 BIO-37085

Proteinase K Solution (20mg/ml)

5 mL

5 x 5 mL

Concentration


Proteinase K is available as a powder or as a stabilized ready-to-use 20 mg/mL (600 mAU/mL) stock solution using a specially formulated calcium acetate buffer.

Storage & Stability

All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.

When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions


On Dry Ice or Blue Ice.