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SensiFAST™ Direct Probe SuperMix is a combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an antibody-mediated hot-start polymerase, dNTPs and MgCl2. It has been designed for highly reproducible, accurate assay results in the presence of inhibitors, making it ideal for direct amplification directly from the most challenging samples.
The advanced buffer chemistry and enhancers has been developed for fast qPCR and is designed for superior sensitivity and specificity with probe‑detection technology, including TaqMan®, Scorpions® and molecular beacon probes, making SensiFAST Direct Probe SuperMix perfect for multiplexing, allowing more samples to be run in a day with the highest confidence, ideal for high‑throughput assays.
Fig. 1 Amplification from different sample types
20% human whole blood containing anticoagulants (EDTA, Heparin and Citrate) and bovine whole blood (EDTA), human cerebrospinal fluid, human urine and cow whole milk were analyses along with 2% alkaline or proteinase K mouse tail, liver and brain crude DNA extracts. The results illustrate that the reaction efficiency of the SensiFAST Direct Probe SuperMix remained within tolerances (90-110%) in the presence of a wide range of common PCR inhibitors.
Fig.2 Amplification from biofluids
A 10-fold serial dilution of genomic DNA spiked into human urine was amplified, using SensiFAST Direct Probe SuperMix (green) and an Inhibitor-Tolerant Mix from supplier K (red) and the manufacturers’ recommended protocol. The results illustrate SensiFAST Direct qPCR Probe SuperMix is more sensitive than the mix from supplier K, as lower dilutions could be detected, with better efficiencies for all three inhibitor samples.
Fig. 3 Amplification from animal DNA in lysate solutions
A 10-fold serial dilution of genomic DNA from mouse tail clippings was spiked proteinase K lysate and after neutralization, used with SensiFAST Direct Probe SuperMix (green) and an Inhibitor-Tolerant Mix from supplier K (red) using the manufacturers’ recommended protocol. The results illustrate SensiFAST Direct qPCR Probe SuperMix is more sensitive than the mix from supplier K, particularly with the proteinase K lysate, as lower dilutions could be detected, with better efficiencies.
Product Name |
500 x 20 µL Reactions |
2000 x 20 µL Reactions |
SensiFAST Probe Direct Supermix (2x) |
5 x 1 mL |
4 x 5 mL |
BIO-86105: 500 x 20 µL Reactions: 5 x 1 mL
BIO-86120: 2000 x 20 µL Reactions: 4 x 5 mL
All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.
On Dry Ice or Blue Ice.