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*link will take you to our exclusive distribution partner site
The SensiMix™ SYBR® Hi-ROX Kit has been developed for highly accurate real-time PCR and has been validated on real-time PCR platforms that require high concentrations of the passive reference dye ROX for normalization of well-to-well differences.
A proprietary, highly-optimized, chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix SYBR® Hi-ROX Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions.
The SensiMix SYBR® Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.
Broad dynamic range SensiMix SYBR Hi-ROX Kit
Amplification curves were obtained for the GAPDH gene over 10 log dilution series of plasmid DNA down to 50 copies. Reactions were in quadruplicate, using standard reaction conditions. The results demonstrate that the kit offers outstanding reproducibility and discrimination even across samples of low copy number templates.
Reduced primer dimer using the SensiMix SYBR Hi-ROX Kit
Melt curve analysis were obtained for GAPDH gene over 10 log dilution series of plasmid DNA down to 50 copies. The presence of one peak indicates that the fluorescence is not attributable to primer-dimers, even in the presence of low template concentrations of template, demonstrating that the kit consistently provides high specificity.
Reagent |
500 x 50 µL Reactions |
SensiMix™ SYBR® No-ROX (2x) |
10 x 1.25 mL (12.5 mL) |
All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.
Shipped on dry/blue ice.