Bioline Core Reagents include a range of high-quality products for routine molecular biology applications, including electrophoresis, cloning and protein digestion.
Bioline electrophoresis reagents include agarose and buffers. The DNase/RNase free agarose is available as powder or pre-weighed tablets and is ideal for high resolution separation of a wide range of DNA/RNA fragments. To complement this are a selection of pre-weighed and ready-to-use running and loading buffers. These products have been specially developed for convenience, efficiency, and to help standardization of nucleic acid and protein electrophoresis.
Bioline offers a range of products to complement our Competent Cells. They consist of ligase, for use in vector construction and X-Gal and IPTG, used in the blue/white selection of transformed colonies.
Proteinase K is an enzyme used to digest most proteins in molecular-biological techniques. The enzyme may be used at 56°C for up to 4 hours, or 37°C for overnight incubations. Proteinase K is available as a powder or as a stabilized stock solution (20 mg/ml). Proteinase K solution is stabilized with a specially formulated buffer and can be used directly from the freezer.
In addition to core reagents for routine molecular biology, Bioline provides reagents for specific detection of bacterial DNA.