| Sign In | Quick Order CART (0)
  • MyTaq™ Polymerases & Mixes

    The MyTaq™ product range is a new generation of very high performance PCR products, designed to deliver outstanding results on all templates, including complex genomic DNA templates.

    MyTaq is based on the latest technology in PCR enzyme preparation, engineered to increase affinity for DNA, resulting in significant improvements to yield, sensitivity and speed. The enzyme is supplied with an industry-leading novel buffer system, containing dNTPs, MgCl2 and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification, making it the perfect choice for complex templates. MyTaq Mix is available as a ready-to-use 2x mix for setting up a trouble-free PCR reaction and there is the choice of an inert red dye, used for direct gel loading.

    • Sensitive – incorporates MyTaq DNA polymerase that exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
    • Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
    • Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
    • Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
    • Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
    • Convenient – includes all the components necessary for high performance PCR amplification

    MyTaq DNA Polymerases Customer Review

    Introduction to MyTaq


    Overview, features and benefits of the MyTaq product family

    My Taq Application Note; Colony PCR

    Superior tolerance of MyTaq to PCR inhibitors in colony PCR

    PCR Enzyme Guide

    Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research

    Fact Sheet

    MyTaq DNA Polymerase

    ““With MyTaq I was able to dramatically boost my efficiencies and increase reproducibility. In addition, I found that multiple primer pairs produced cleaner products with improved melting curves.”

    UC Davis School of Veterinary Medicine, USA

    MyTaq™ HS Polymerases & Mixes

    MyTaq™ HS is a new generation of very high performance, antibody-mediated hot-start PCR products, designed to give outstanding results with complex genomic DNA templates.

    MyTaq HS uses the latest technology in PCR enzyme design, engineered to increase affinity for DNA, so resulting in significant improvements in yield, sensitivity and speed. The product also has the added convenience of room temperature reaction assembly, without non-specific amplification and primer-dimer formation. The enzyme is supplied with an industry-leading novel buffer system, containing dNTPs, MgCl2 and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification, making it the perfect choice for complex templates. MyTaq HS Mix is available as a ready-to-use 2x mix for setting up a trouble-free PCR reaction and there is the choice of an inert red dye, used for direct gel loading.

    • Sensitive – incorporates MyTaq HS DNA Polymerase which exhibits increased affinity for DNA; thereby improving amplification of even limiting amounts of template
    • Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
    • Specific – MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
    • Robust – reliable amplification in the presence of inhibitors and with even the most challenging DNA targets
    • Flexible – ideal for amplifying any target up to 5 kb from DNA extracted from human, animal and plant samples
    • Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast conditions

    PCR Selection Chart

    Select the best reagent for your research

    Fact Sheet

    MyTaq HS DNA Polymerase

    MyTaq™ One-Step RT-PCR Kits

    The MyTaq™ One-Step RT-PCR Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system ensures that MyTaq One-Step RT-PCR Kit produces fast, highly-specific and ultra-sensitive one-step RT-PCR, perfect for all downstream applications. MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and a potent RNase Inhibitor, RiboSafe, that are added together to create a simple to use all-in-one mix.

    • Sensitive – incorporates a blend of high-affinity RT and novel MyTaq HS DNA Polymerase, enabling amplification of low copy number targets from ≥3 pg total RNA
    • Efficient – novel one-step buffer system maximizes the efficiency of both the reverse transcription and PCR steps, delivering improved yield of any target
    • Robust – RT tolerates the higher reaction temperatures required to overcome secondary structure, giving reliable detection of even challenging and GC-rich targets
    • Specific – MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
    • Flexible – utilizes gene-specific primers for full-length reverse transcription and subsequent PCR amplification of any RNA target
    • Convenient – an all-in-one-tube mastermix that improves the speed, convenience and accuracy of RT-PCR

    “When we compared the performance of our routine supplier’s RTase against Bioline’s MyTaq One-Step RT-PCR, the other supplier’s RTase gave two false negatives in five different grapevine samples tested for Grapevine rupestris stem-pitting-associated Foveavirus. We were convinced to immediately switch.”

    University of Adelaide, Australia

    MyTaq™ Direct PCR Kits

    Bioline direct-PCR kits deliver unparalleled convenience for DNA amplification by allowing PCR directly from crude samples. Based on MyTaq™ HS DNA Polymerase the kits have been specifically engineered to overcome PCR inhibitors typically present in crude samples and allow fast cycling conditions to be used, without compromizing PCR specificity and yield. High sensitivity and specificity also make direct-PCR kits highly-suited to multiplex PCR and high-throughput genotyping assays from small amounts of source material, generating significant savings in both time and cost.

    • Fast - eliminate complex, slow and costly DNA extraction steps, thereby reducing time to results
    • Sensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA; thereby improving yield of even the most challenging targets
    • Simple – fewer protocol steps greatly reduces the risk of sample loss and contamination and minimizes manual effort
    • Robust - novel buffer system developed to overcome inhibition for highest PCR success rates and improved sensitivity
    • Flexible - developed for a wide range of blood, tissue and plant samples, thereby avoiding the need for further optimization
    • Versatile - developed for a range of PCR applications, including multiplexing, amplification of GC rich templates and long amplicons

    MyTaq Plant-PCR Kit Workflow

    Dr. Steve Hawkins delivers a simple protocol for Direct Plant PCR using MyTaq Plant-PCR Kit.

    MyTaq Blood-PCR Kit Customer Review

    “I’ve tested a number of products and by far the best one was MyTaq Blood-PCR Kit. The major advantage is that it works on blood samples and crude lysates. This product will also amplify larger amplicons than other products tested, even in a multiplex scenario. Other key advantages include the hot-start and its ability to withstand 30 freeze-thaw cycles.”

    Markus Zeller, AutoGenomics, California, US

    Reviews

    "MyTaq Extract-PCR kit was effective and efficient for high throughput ..."

    "We have been using MyTaq Red Mix for the past two years in our ..."

    "We use MyTaq DNA Polymerase for our mouse ..."

    "MyTaq HS Mix (BIO-25045) works great even in samples where other PCR ..."

    "I like this product because it is easy to use and fast to set up a PCR ..."

    "I use the MyTaq HS Red Mix all the time for genotyping purposes ..."