Oligo (dT)18 Primer is single-stranded sequence of deoxythymine (dT), used for priming reactions catalysed by reverse transcriptase. The transcript is primed in the poly(A) tail of mRNA molecules.
- Long oligonucleotide -more thermostable Reverse Transcriptases perform better with longer primers
- Binds only to mRNA -can be used for cDNA synthesis with total RNA
Oligo (dT)18 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented.
Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´
A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.
- cDNA synthesis with a reverse transcriptase
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Certification of Analysis (COAs)
Oligo dT (270 ng/µL)
1 x 100 µL
100 µL at 270 ng/µL
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
Oligo (dT)18 is shipped on dry/blue ice