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    Ready-to-use molecular weight marker, designed for easy size determination of nucleic acids from 25 bp to 500 bp.

    Product Highlights

    • Broad size range – 12 evenly distributed bands from 25 bp to 500 bp
    • Easy to orientate – several brighter reference bands
    • Stable – can be stored at room temperature for up to 6 months
    • Supplied with loading dye – for loading sample DNA

    Product Description

    HyperLadder™ 25bp is a molecular weight marker, especially designed for easy size determination of linear double-stranded DNA fragments on 2% to 3% TAE or TBE agarose gels or polyacrylamide gels.

    The 10 regularly spaced bands, ranging from 25 bp to 500 bp, with higher intensity reference bands at 100 bp and 200 bp allow for easy identification and orientation of your band at a glance.

    This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 25bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 25bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 25bp is also supplied with a 5x loading dye for loading of sample DNA.

    HyperLadder 25bp is perfect for size determination in techniques that produce very short fragments such as apoptotic DNA fragmentation as well as other downstream techniques.


    • Apoptotic DNA fragmentation
    • Restriction fragment mapping
    • Confirmation of plasmid construction
    • Detection of DNA polymorphisms
    • Southern/Northern blotting
    • Cloning
    Ready-to-use molecular weight marker for easy size determination.




    200 Lanes

    500 Lanes

    HyperLadder™ 25bp

    2 x 500 µL

    5 x 500 µL

    5x Sample Loading Buffer

    1 mL

    1 mL

    Storage & Stability

    All components are shipped at room temperature or on dry/blue ice and should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date on the outer box label.

    Components may also be stored at +4°C or room temperature if required, although storage at -20°C is always recommended.

    When stored at +4°C, reagents will remain stable for a period of 12 months.

    When stored at room temperature, reagents will remain stable 6 months from date of receipt.

    Shipping conditions

    At ambient temperature.


    All HyperLadders and EasyLadders are ready-to-load and require no addition of loading buffer prior to use. In general, the smaller the DNA product, the higher the percentage of Agarose required in the gel. As a guide for optimization we would suggest to use the EasyLadder I with 2% (w/v) agarose gel,

    Hyperladder 100bp with 2% (w/v) agarose gel,

    Hyperladder 1kb with 1% (w/v) agarose gel,

    Hyperladder 50bp with 1.5% (w/v) agarose gel and

    Hyperladder 25bp with 3.5% (w/v) agarose gel.

    Please have a look into the related application note for more information about ladder migration in different gel concentrations.

    Problems with our HyperLadder and EasyLadder are extremely rare, so please make sure you have carried out each of the following correctly:

    -          Used the correct percentage of agarose in your gel for the specific marker in question.

    -          Used 5 µL of the marker per lane.

    -          Used the correct concentration of stain (e.g. EtBr) to visualise correctly.

    -          Vortexed the marker gently before use.

    Should problems persist, please contact technical services for further assistance.

    No pre-treatment, such as denaturing, is necessary prior to use.

    No, our Ladder range is supplied in a ready-to-load format to be directly loaded onto your agarose gel. An additional vial of 5x Loading Buffer is supplied with each of our markers for use with your samples.

    Our HyperLadder and EasyLadder range is designed for use on Agarose gels and we do not recommend them for PAGE. However, should you wish to use Bioline HyperLadders in this technique, HyperLadder 25bp, our small fragment DNA marker, would be the most appropriate.

    Other stains, such as SYBR® Green Gel Stain, can be used with our ladders as effectively as EtBr. The fluorescence of the gel as a whole may vary depending on the stain used, however the relative intensities of the marker bands and of the samples run will remain the same, thus the HyperLadder / EasyLadder remains quantitative.

    EasyLadder I: gel with 2% (w/v) agarose in TBE

    HyperLadder 1kb: gel with 1% (w/v) agarose in TBE

    HyperLadder 100bp: gel with 2% (w/v) agarose in TBE

    HyperLadder 50bp: gel with 1,5% (w/v) agarose in TBE

    HyperLadder 25bp: gel with 3,5% (w/v) agarose in TBE

    For all 5 µL ladder have been applied and visualized using ethidium bromide.