- Sensitive – exhibits increased affinity for DNA, thereby improving amplification of even limiting amounts of template
- Efficient – novel buffer system maximizes efficiency of PCR amplification, delivering improved yield of any PCR product
- Specific – an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplification
- Flexible – ideal for amplifying any target up to 5 kb, including DNA extracted from human, animal and plant samples
- Fast – developed to give sensitive, reproducible and robust amplification of a broader range of targets under fast thermal cycling conditions
- Convenient – includes all the components necessary for high performance PCR amplification
MyTaq™ HS Red DNA Polymerase is a new generation of antibody-mediated hot-start enzyme, engineered for highly specific and efficient amplification from even the most challenging templates. MyTaq HS remains inactive at room temperature allowing for convenient reaction set-up, thereby reducing non-specific amplification that can hinder PCR assays from the start. These properties make MyTaq HS Red DNA Polymerase the ideal choice for PCR assays containing complex and low copy number targets, as well as multiplex PCR.
The composition of the buffer system is critical for efficient PCR. MyTaq reaction buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps give more robust amplification than other commonly-used polymerases, meaning it performs reliably even in the presence of PCR inhibitors, permitting amplification of longer fragments.
The combination of MyTaq and optimized buffer system allow for faster PCR reactions compared with other polymerases, therefore reducing overall run time from approximately 1 hour to under 30 minutes. This is achieved without compromising specificity or yield, reducing the reaction time allows for increased throughput and faster time to results.
MyTaq HS Red DNA Polymerase includes a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR eliminating the need to add loading buffer.
- Fast PCR
- Multiplex PCR
- Specific amplification of challenging (e.g. GC-rich) templates
- Multiplex PCR
- Colony PCR
- Low copy number PCR assays
- High-throughput assays with prolonged PCR set-up
Introduction to MyTaq
Overview, features and benefits of the MyTaq product family
PCR Selection ChartSelect the best reagent for your research
PCR Enzyme GuideDownload the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research
Application NoteMyTaq™ HS Red DNA Polymerase
I like this product because it is easy to use and fast to set up a PCR reaction. Very convenient! I used it for regular PCR and it worked well to amplify my product. It also has a red mix, so after PCR I don't have to add any loading buffer, which is awesome!
Kristina Marinak, West Virginia University, Morgantown, US
Product SelectionPlease refer to the PCR Selection Chart to confirm the recommended product for your PCR application.
Certification of Analysis (COAs)
MyTaq HS Red DNA Polymerase
1 x 200 µL
2 x 250 µL
5x MyTaq Reaction Buffer
8 x 1 mL
14 x 1.5 mL
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
On Dry Ice or Blue Ice.
One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C.
MyTaq is a trademark of Bioline.
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
|No or low PCR yield||Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments.|
|Primers degraded – check quality and age of the primers.|
|Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM.|
|Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration.|
|Template concentration too low – Increase concentration of template.|
|Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated.|
|Multiple Bands||Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers.|
|Prepare master mixes on ice or use a heat-activated polymerase.|
|For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity.|
|Smearing or artifacts||Template concentration too high. Prepare serial dilutions of template.|
|Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands.|
|Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments.|
|Extension time too long. Reduce extension time in 0.5-1 minute increments.|