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Random Hexamer Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers and cDNA synthesis.
Random Hexamer Primers consist of a mixture of oligonucleotides representing all possible hexamer sequences. Random Hexamer Primers are commonly used for priming single-stranded DNA or RNA for extension by DNA polymerases or reverse transcriptases.
During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between mRNA and other RNA species present in the reaction.
Primer Sequence: 5´ – d (NNNNNN) –3´ N = G, A, T or C
A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.
|
Reagent |
Volume |
|
Random Hexamer Primers (50 ng/µL) |
1 x 500 µL |
500 µL at 50 ng/μL
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
Shipped on dry/blue ice.
