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Description
Product Highlights
- Efficient – specifically developed to give reliable amplification of fragments between 10 kb and 25 kb in length
- Specific – incorporates an antibody-mediated hot-start enzyme blend that remains completely inactive during PCR set-up to prevent non-specific amplification
- Fast – rapid enzyme activation and highly efficient amplification support reduced time to results
- Flexible – ideal for amplifying a broad range of large fragments from even complex targets including human genomic DNA
- Accurate – possesses 3’ – 5’ proofreading exonuclease activity for increased fidelity, thereby enabling cloning of long PCR products
Product Description
RANGER DNA Polymerase is recommended for all long PCR applications. RANGER is an easy-to-use, high performance enzyme blend, specifically developed to amplify fragments up to 25 kb in length. RANGER contains a unique combination of a high-performance DNA polymerases and novel buffer system that deliver the improved efficiency necessary for reliable amplification of longer amplicons.
RANGER is an antibody-mediated hot-start enzyme blend that eliminates non-specific amplification during reaction set-up. The inactivated enzymes do not possess polymerase activity, thereby preventing the non-specific amplification, such as primer-dimer formation, that often hinder long PCR reactions from the start.
RANGER is provided with a specially formulated reaction buffer containing dNTPs, MgCl2 and enhancers at optimal concentrations, minimizing the requirements for PCR optimization, in turn reducing time to results and eliminating the cost of unnecessary repeats.
Applications
- Long PCR
- TA cloning
- Higher fidelity PCR amplification
PCR Enzyme Guide
Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your researchPCR Selection Chart
Select the best reagent for your researchHaving struggled with PFU and other polymerases, I was extremely happy to try RANGER DNA Polymerase and it worked for both 8.5 kb and 6.5 kb.
NWCR Institute, Bangor University, UK
Product Selection
Please refer to the PCR Selection Chart to confirm the recommended product for your PCR application.Resources
Documents
Certification of Analysis (COAs)
Specification
Components
|
Reagent |
250 Units |
500 Units |
|
RANGER DNA Polymerase |
1 x 62.5 µL |
1 x 125 µL |
|
5x RANGER Reaction Buffer |
1 x 1.2 mL |
1 x 1.2 mL |
Volume
- BIO-21121: 62 x 50 µL Reactions: 1 x 62.50 µL
- BIO-21122: 125 x 50 µL Reactions: 1 x 125 µL
Concentration
- 4 u/µL
Storage & Stability
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
RANGER DNA Polymerase may also be stored at +4°C for up to 2 weeks, although storage at -20°C is recommended.
Shipping conditions
On Dry Ice or Blue Ice.
FAQs
Please Note: We do not recommend the storage of our polymerases at -80 °C as ice crystals could form on the active site, which may affect or destroy the activity of the enzyme.
| Observation | Recommended Solution(s) |
| No or low PCR yield | Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments. |
| Primers degraded – check quality and age of the primers. | |
| Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM. | |
| Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration. | |
| Template concentration too low – Increase concentration of template. | |
| Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated. | |
| Multiple Bands | Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers. |
| Prepare master mixes on ice or use a heat-activated polymerase. | |
| For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity. | |
| Smearing or artifacts | Template concentration too high. Prepare serial dilutions of template. |
| Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands. | |
| Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments. | |
| Extension time too long. Reduce extension time in 0.5-1 minute increments. |
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