A unique blend of high-performance hot-start MyTaq™ HS DNA Polymerase and a proprietary proofreading enzyme, specifically developed for long PCR applications
RANGER DNA Polymerase is recommended for all long PCR applications. RANGER is an easy-to-use, high-performance enzyme blend, specifically developed to amplify fragments up to 25 kb in length. RANGER contains a unique combination of a highly-efficient DNA polymerases and novel buffer system that deliver the improved efficiency necessary for reliable amplification of longer amplicons.
RANGER is an antibody-mediated hot-start enzyme blend that eliminates non-specific amplification during reaction set-up. The inactivated enzymes do not possess polymerase activity, thereby preventing the non-specific amplification, such as primer-dimer formation, that often hinder long PCR reactions from the start.
RANGER is provided with a specially formulated reaction buffer containing dNTPs, MgCl2 and enhancers at optimal concentrations, which deliver increased processivity, sensitivity and specificity, thereby enabling successful amplification of long human genomic DNA fragments. This minimizes the requirements for PCR optimization, in turn reducing time to results and inconvenience of performing unnecessary repeats.
|
Reagent |
250 Units |
500 Units |
|
RANGER DNA Polymerase |
1 x 62.5 µL |
1 x 125 µL |
|
5x RANGER Reaction Buffer |
1 x 1.2 mL |
1 x 1.2 mL |
All components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
RANGER DNA Polymerase may also be stored at +4°C for up to 2 weeks, although storage at -20°C is recommended.
On Dry Ice or Blue Ice.
| Observation | Recommended Solution(s) |
| No or low PCR yield | Enzyme concentration too low – increase the amount of enzyme in 0.5 U increments. |
| Primers degraded – check quality and age of the primers. | |
| Magnesium concentration too low – increase concentration in 0.25 mM increments with a starting concentration of 1.75 mM. | |
| Primer concentration not optimized. Titrate primer concentration (0.3-1 µM); ensuring that both primers have the same concentration. | |
| Template concentration too low – Increase concentration of template. | |
| Perform a positive control to ensure that the enzyme, dNTPs and buffers are not degraded and/or contaminated. | |
| Multiple Bands | Primer annealing temperature too low. Increase annealing temperature. Primer annealing should be at least 5°C below the calculated Tm of primers. |
| Prepare master mixes on ice or use a heat-activated polymerase. | |
| For problems with low specificity. Try adding 3% DMSO (not supplied) to improve specificity. | |
| Smearing or artifacts | Template concentration too high. Prepare serial dilutions of template. |
| Too many cycles. Reduce the cycle number by 3-5 to remove non-specific bands. | |
| Enzyme concentration too high - decrease the amount of enzyme in 0.5 U increments. | |
| Extension time too long. Reduce extension time in 0.5-1 minute increments. |
Please click here in order to request your sample. You will receive an email confirmation within two business days with delivery details.
