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    Cat. No.
    Size
    BIO-84005
    500 x 20µl Reactions
    BIO-84020
    2000 x 20µl Reactions
    BIO-84050
    5000 x 20µl Reactions

    *link will take you to our distributor which supports all Academic and Government Institutes. All other customers please submit your order to orders@meridianlifescience.com

    Description

    Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.

    Product Highlights

    • Reproducible – consistent results between technical replicates for increased confidence in results
    • Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types
    • Sensitive – reliable quantification of low abundance targets and scarce samples
    • Fast – delivers reproducible, accurate assay results in as little as 30 minutes
    • Efficient – excellent performance in multiplex assays
    • Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability

    Product Description

    The SensiFAST™ Probe Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.

    A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays.

    The SensiFAST Probe Lo-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

    Applications

    • Gene expression analysis
    • DNA / RNA target detection
    • Copy number variation (CNV) analysis
    Main
    Highly efficient under fast conditions.

    Introduction to SensiFAST

    Overview, features and benefits of the SensiFAST product family

    Real-Time PCR Selection Chart

    One-step Vs. Two-step real-time RT PCR

    A discussion of the pros and cons of each detection strategy.

    Application Note

     SensiFAST™ Probe Lo-ROX Kit

    I performed a comparison of Bioline's SensiFAST™ Probe against several other qPCR master mixes. The Bioline product performed the best overall in terms of efficiency, sensitivity, lower in cost. I have also been very impressed Bioline's customer service.

    Tiffany Kosch, James Cook University, Townsville, Australia

    Instrument Compatibility

    Please refer to the Real-Time PCR Selection Chart to confirm the SensiFAST Probe Lo-ROX Kit is compatible with your instrument.


    Resources

    Documents

    Employing Cell-free DNA from Maternal Plasma for Exclusion of X-linked Disorders SensiFAST Probe Lo ROX Kit - Manual SensiFAST Probe Lo-ROX - MSDS SensiFAST qPCR Guide

     

    Real-Time PCR Selection Chart One-step Vs. Two-step real-time RT PCR

    Certification of Analysis (COAs)

    BIO-84050_SF886-B078650 BIO-84005_SF590-B077980 BIO-84005_SF590-B078140 BIO-84050_SF886-B076590 BIO-84020_SF591-B076990

    ViewHide all 85 COAs

    Specification

    Components

    Reagent

    500 x 20 µL Reactions

    2000 x 20 µL Reactions

    5000 x 20 µL Reactions

    SensiFAST Probe Lo-ROX Mix (2x)

    5 x 1 mL

    4 x 5 mL

    10 x 5 mL

     

    Volume

    BIO-84005: 500 x 20 µL Reactions: 5 x 1 mL
    BIO-84020: 2000 x 20 µL Reactions: 4 x 5 mL
    BIO-84050: 5000 x 20 µL Reactions: 10 x 5 mL

    Storage & Stability

    All kit components should be stored at -20°C upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label.

    Avoid exposure of the ROX™ to light.

    Shipping conditions

    Shipped on dry/blue ice.



    FAQs

    Can I use a SensiFAST SYBR Kit for a probe assay?

    This is not possible because the SYBR® is pre-mixed into the SensiFAST SYBR® mastermix.

    What template (RNA/cDNA) is this compatible with?

    SensiFAST One-Step Kits can be used with most RNA/cDNA templates. To give you some idea of a few of the types of templates used with these kits:

    Human - Rosato R.R., et al. Cancer Res. 67: 9490-500 (2007)
    Rat - Remund K., et al. Expt. Lung Res. 35: 359-370 (2009)
    Drosophila - Brown A.E., et al. PloS ONE 4: e4490 (2009)
    Nitrogen-fixing bacteria - Bahlawane C., et al. Mol. Plant-Microbe Inter. 21: 1498-1509 (2008)
    Diphtheria bacteria - Jochmann N., et al. Microbiology 155: 1459-1477 (2009)
    Green algae - Wobbe L., et al. PNAS 106: 13290-13295 (2009)
    Bovine virus - Park S-I., et al. J. Virol. Methods. 159: 64-6 (2009)

    For cDNA/DNA templates SensiFAST SYBR® and Probe kits are used. To give you some idea of a few of the types of templates used with these kits:

    Stem Cell - Bernardo A.S., et al. Stem Cells 27(2): 341 -351 (2008)
    Cow - Baumert A., et al. J. Dairy Research 76(3): 356-364 (2009)
    Mouse - Hoyles R.K., et al. Arthritis Rheum. 58(4): 1175-88 (2008)
    Quail - De Winter P., et al. British Poultry Science 49(5): 566 – 573 (2008)
    Insect - Bass C., et al. Malaria journal 6 111 (2007)
    Fish - Miller M.R., et al. J. Nutr. 138(11): 2179-2185 (2008)
    Crab - Wilcockson D.C. & Webster S.G. Gen. & Comp. Endo. 156: 113–125 (2008)
    Nematode - Murray S.L., et al. Mol. Plant-Microbe Interactions 20(11): 1431–1438 (2007)
    Plant - Hecht V., et al. Plant Physiology Preview DOI:10.1104/pp.107.096818 (2007)
    Yeast - Kawauchi J., et al. Genes & Dev. 22: 1082-1092 (2008)
    E.coli Bacteria - Saeed H.A., et al. Research J. Microbiology 4(4); 173-177 (2009)
    Virus - Muscillo M., et al. Water, Air, & Soil Pollution 191: 1-4 (2008)


    Can I use a SensiFAST Kit for standard real-time PCR?

    Although SensiFAST kits have been designed for fast PCR on the new generation of fast machines, they will work equally well for standard or fast PCR protocols on all real-time PCR machines.

    Why do the SensiFAST kits contain a hot-start Taq polymerase?

    Polymerase activity during the reaction set-up causes non-specific amplification including primer-dimer formation. To avoid non-specific amplification the polymerase is only activated after heating at 95°C for 2-3 minutes.

    I would like to compare SensiFAST with my current qPCR mix. What do I have to consider?

    When comparing SensiFAST with a mix from another supplier we strongly recommend to use the suggested conditions for PCR setup and cycling. Every real-time mix has its specific composition and different properties. That’s why they should be tested with their specific conditions, mostly this is possible on different plates only. We recommend amplifying from a ten-fold template dilution series. Loss of detection at low template concentration is the only direct measurement of sensitivity. An early Ct value is not an indication of good sensitivity, but rather an indication of speed.

    I would like to use the SensiFAST Probe mix with hybridization probes instead of TaqMan, what do I have to consider?

    The recommended PCR conditions in the manual refer to the set-up of TaqMan probe-based PCR. If you want to use other probe types, it may be necessary to adapt the PCR setup conditions, please refer to the relevant literature. In case of hybridization probes it would be necessary to switch to a three-step PCR setup with annealing at 60°C and extension at 72°C and acquire the data at the end of the annealing phase (the exact annealing temperature may vary and has to be optimized for a specific assay). The two-step protocol with an annealing / extension step at 60°C would lead to the degradation of these probes, which is wanted for TaqMan but not for hybridization probes. If you have further questions, please contact Bioline Technical Support.

    Why do certain kits contain a ROX?

    The emission recorded from ROX during the baseline cycles is used to normalize the emission recorded from the reporter (SYBR®) in later cycles in some instruments. ROX compensates for small fluorescent fluctuations such as bubbles and well-to-well variations that may occur.

    Why are the ROX concentrations different?

    The amount of the ROX passive reference dye needed varies depending on the instrument optics. Our SensiFAST kits have been optimized for all these different instruments (see Product Selection Tool).

    What is the advantage of working with SYBR Green I?

    SYBR® Green I is an inexpensive, universal dye which binds to all dsDNA. It can be easily used in combination with a simple primer pair to detect PCR products in real-time. This dye is very attractive for researchers analysing lots of different genes.

    What is the advantage of working with a probe system?

    The probe system is always specific, probes only detect the gene of interest. It is also possible to distinguish between similar sequences with small differences like SNPs or mutations. In general, probe assays need less optimization than SYBR® Green I assays.

    Why do you not sell kits contain dUTP (and UNG)?

    This method is only effective if all the researchers in the laboratory, or using the thermocycler, are all using a dUTP/dNTP and UNG system. If even one researcher is not, it ceases to be an effective control. Using dUTP has also been shown to be inefficient as it increases the Ct values by reducing reaction efficiency. Most labs do not need to use the dUTP method of control, optimised protocols will allow high specificity of PCR and good lab practices (using disposable consumables, the use of filter tips and maintaining a separate area for PCR set-up and PCR amplification and any post-PCR analysis) virtually eliminate the risk of cross contamination.

    What is the difference between a one-step and a two-step real-time PCR reaction?

    In a one-step reaction the reverse transcription reaction and the real-time PCR reaction are done in one tube, making this a closed tube assay, so contamination can be avoided. It saves pipetting steps and time and is easy in handling, making it ideal for high throughput screening. In a two-step reaction the reverse transcription reaction and the real-time PCR reaction are done in separate tubes. It gives a more flexible way of working in that the cDNA can be used for more than one real-time PCR reaction and can be archived, eliminating the need to continually isolate RNA. For convenience Bioline sells the SensiFAST cDNA Synthesis Kit separately if you wish to take a two-step approach.

    What could be the reason of strange shaped qPCR curves? I observed an early increase of the signal and strange shaped qPCR curves without an initial plateau.

    A possible reason is that the template amount is too concentrated. An early Ct may impair the baseline correction and may cause these strange curves. We suggest to dilute the template. Alternatively if the curve is flattened, this may be due to inhibitors in the reaction. Running a serial dilution of your sample will dilute the inhibitor concentration, if this corrects the problem, you will either need to go back and re-purify your template, or use it at a lower concentration, where the inhibitor effect has been diluted out.

    Reviews

    "We recently trialled Bioline's SensiFAST cDNA Synthesis Kit and the ... "

    ""For SensiFAST Probe Lo-ROX Kit “Overall, the reagent is good and ... "

    Citations

    1

    Molecular Detection of HIV-1 Subtype B, CRF01_AE, CRF33_01B, and Newly Emerging Recombinant Lineages in Malaysia

    Chook, J.B., Ong, L.Y., Takebe, Y., Chan, K.G., Choo, M., Kamarulzaman, A., Tee, K.K.     2

    A Vasoactive Role for Endogenous Relaxin in Mesenteric Arteries of Male Mice

    Leo, C.H., Jelinic, M., Gooi, J.H., Tare, M., Parry, L.J.     3

    Development and application of a quantitative real-time PCR assay for the globally invasive tunicate Styela clava

    Gillum, J.E., Jimenez, L., White, D.J., Goldstien, S.J., Gemmell, N.J.


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