α-Select Gold Competent Cells are ideal for generating high efficiency libraries cloning with limited amounts of DNA.
- Reduced recombination of cloned DNA - accommodates larger plasmids
- High efficiency - available in ≥109 cfu/μg, perfect for library construction
- Easy screening - with blue/white color selection
- Convenient size - small aliquots reduce waste
α-Select Gold Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors.
α-Select Gold Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.
pUC19 DNA is also provided as a positive control.
- Transformation of large plasmids into bacterial cells
- Generating cDNA libraries
- Blue/white color screening
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Certification of Analysis (COAs)
Chemically Competent Cells
20 x 50 µL
pUC19 control vector (10 pg/µL)
Storage & Stability
All components should be stored at -80°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
Shipped on dry ice.