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  • Hints, Tips & Tricks - Annealing Temperature

    February 17, 2016

    Test a range of annealing temperatures. Depending on the qPCR results, the annealing temperature should be increased or decreased in 2-3oC increments. This can be done in a single experiment using a thermal gradient. Alternatively, a range of annealing temperatures should be tested using multiple qPCR experiments.

    Posted in: BiolinePCR Tags: qPCRAnnealing Temperature