The SensiFAST SYBR® Lo-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube.
An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.
The SensiFAST SYBR® Lo-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX.
|
Reagent |
100 x 20 µL Reactions |
500 x 20 µL Reactions |
|
SensiFAST SYBR® Lo-ROX One-Step Mix (2x) |
1 x 1 mL |
5 x 1 mL |
|
RiboSafe RNase Inhibitor |
1 x 40 µL |
1 x 200 µL |
|
Reverse transcriptase |
1 x 20 µL |
1 x 100 µL |
|
DEPC-H2O |
1 x 1.8 mL |
2 x 1.8 mL |
All kit components should be stored at -20°C upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR® Green I to light.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.
Shipped on dry/blue ice.
